Active Calmodulin (CAM)

CALcium MODULated ProteIN

ACTIVITY TEST

Calmodulin (CAM) is the archetype of the family of calcium-modulated (calmodulin) proteins of which nearly 20 members have been found. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Besides, Myosin IC (MYO1C) has been identified as an interactor of CAM, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAM and recombinant human MYO1C. Briefly, CAM were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to MYO1C-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAMpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CAM and MYO1C was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of CAM with MYO1C.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One. Identification of Novel Translational Urinary Biomarkers for Acetaminophen-Induced Acute Liver Injury Using Proteomic Profiling in Mice PubMed: PMC3498140
PLOS ONE Role of Calmodulin-Calmodulin Kinase II, cAMP/Protein Kinase A and ERK 1/2 on Aeromonas hydrophila-Induced Apoptosis of Head Kidney Macrophages Pubmed:24763432
PLoS One Calcium and Superoxide-Mediated Pathways Converge to Induce Nitric Oxide-Dependent Apoptosis in Mycobacterium fortuitum-Infected Fish Macrophages Pubmed:26752289
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA640Hu01 Recombinant Calmodulin (CAM) Positive Control; Immunogen; SDS-PAGE; WB.
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SEA640Hu ELISA Kit for Calmodulin (CAM) Enzyme-linked immunosorbent assay for Antigen Detection.