Active Adiponectin (ADPN)

GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

ACTIVITY TEST

Human Adiponectin (ADPN) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is a plasma protein secreted by adipose tissue showing pleiotropic effects with anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin and AdipoR1 regulate PPARgC1a and mitochondria by Ca(2 ) and AMPK/SIRT1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant chicken ADPN and recombinant rat PPARgC1a. Briefly, ADPN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARgC1a-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant chicken ADPN and recombinant rat PPARgC1a was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA605Ga01 Recombinant Adiponectin (ADPN) Positive Control; Immunogen; SDS-PAGE; WB.
APA605Ga01 Active Adiponectin (ADPN) Cell culture; Activity Assays.
PAA605Ga01 Polyclonal Antibody to Adiponectin (ADPN) WB; IHC; ICC; IP.
MAA605Ga21 Monoclonal Antibody to Adiponectin (ADPN) WB; IHC; ICC; IP.
SEA605Ga ELISA Kit for Adiponectin (ADPN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA605Ga CLIA Kit for Adiponectin (ADPN) Chemiluminescent immunoassay for Antigen Detection.
LMA605Ga Multiplex Assay Kit for Adiponectin (ADPN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.