Active Matrix Metalloproteinase 9 (MMP9)

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

ACTIVITY TEST

Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant human MMP9 (1000ng, 500ng, 100ng, 10ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant human MMP9 (10-70kd) was shown in figure 1.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Bone and Joint Surgery Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180
Journal of Surgical Research Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia ScienceDirect: S0022480409001942
Journal of Proteome Research The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9 ACS: pr2006395
Zahedan Journal of Research in Medical Sciences The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men Zjrms: Source
Experimental & Translational Stroke Medicine Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435
PLoS One Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Rheumatology International Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat model Pubmed: 23408150
Clinical and Investigative Medicine The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells Pubmed: 23544611
Acta Histochemica RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells Pubmed: 24681113
International journal of colorectal disease Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair Pubmed: 23619615
Journal of Periodontology Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial Joponline: 130464
Polski Przeglad Chirurgiczny Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Int J Clin Exp Med. Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model Pubmed:Pmc4132129
The Scientific World Journal Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats Pubmed:24701154
Journal of Perinatology Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion Pubmed:25078863
World J Emerg Med Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9 Pubmed:25215164
PLOS ONE Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat Model Pubmed:25314292
Stem Cells International Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Pubmed:25767513
Physiology and Pharmacology A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men Phypha:Source
Mediators of Inflammation Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi
Journal of Biomaterials and Tissue Engineering MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 Content: Asp
PLOS Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat PubMed: 25945502
Biomedical Materials In vivo PubMed: 25634132
Histol Histopathol Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells PubMed: 26254457
Int J Clin Exp Pathol Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways PubMed: 26191138
Hattori and Ishihara Stem Cell Research & Therapy Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells PubMed: 25884474
Neuropeptides Melanocortin MC5 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140
Canadian Journal of Physiology and Pharmacology Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats Doi: Abs
PLASTIC AND AESTHETIC RESEARCH A comparative study to evaluate the effect of limited access dressing on diabetic ulcers Article: 2347-9264
Kyselov Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого … Article: View
Оригинальные исследования / Original researches матриксу та ранн Article: Viewfile
Int J Mol Sci Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ PubMed: 26703570
Deletion Polymorphism and Chronic Obstructive Pulmonary Disease ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970
Basic Research CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699
Journal of Cellular and Molecular Medicine Chronic myelogenous leukaemia exosomes modulate bone marrow microenvironment through activation of epidermal growth factor receptor Pubmed:27196940
Journal of Stroke and Cerebrovascular Diseases Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats Pubmed:26851971
Journal of the Science of Food and Agriculture Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells Pubmed:26991449
Cell Physiol Biochem. MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 Pubmed:27160777
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
Onco Targets Ther. sequential measurements of serum matrix metalloproteinase 9 to monitor chemotherapy responses in patients with advanced non-small-cell lung cancer pmc:PMC4898419
Am J Pathol.  Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343
Biochem Pharmacol. Gallium, a promising candidate to disrupt the vicious cycle driving osteolytic metastases. pubmed:27378505
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451
Balkan Medical Journal Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage pubmed:27761275
SpringerPlus The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … pubmed:27652141
Cerebrovascular Diseases Extra Increased Plasma Matrix Metalloproteinase-9 Levels Contribute to Intracerebral Hemorrhage during Thrombolysis after Concomitant Stroke and Influenza Infection pubmed:27560521
Microvascular Research Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518
BMC Veterinary Research Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355
Cellular Physiology and Biochemistry MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. pubmed:27160777
Journal of Biomaterials and Tissue Engineering MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line publication:306067745
J Sci Food Agric. Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells. pubmed:26991449
Sci Total Environ. The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. pubmed:28174048
Journal of Biomaterials and Tissue Engineering Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts art00008
Human cell Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity pubmed:28091828
Human Cell MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 pubmed:27743351
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422
Human Cell MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 pubmed:28573504
Cellular Physiology and Biochemistry The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019
Evidence-Based Complementary and Alternative Medicine Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
Fundamental & Clinical Pharmacology Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 DOI:10.1111/fcp.12294
Scientific Reports Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress doi:10.1038/s41598-017-06535-y
Scientific Reports Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway pubmed:28600504
Archives of Medical Science CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. pubmed:28507585
Molecular Medicine Reports Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis pubmed:28901438
Surgical Innovation A Time Course of Bevacizumab (Anti-VEGF) Effect on Rat Peritoneum: Relations Between Antiadhesive Action and Fibrin Regulation Enzymes. pubmed:28877644
Journal of Neurochemistry Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling Pubmed:29572844
Journal of Cellular Biochemistry Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia … 10.1002:jcb.26849
Inflammation Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy Pubmed:29704151
OncoTargets and Therapy sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion Pubmed:30022839
Biomed Research International CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling Pubmed:29984229
Oncology Letters miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells 10.3892:ol.2018.9170
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM Pubmed:29673663
Journal of Veterinary Science Clinical assessment after human adipose stem cell transplantation into dogs Pubmed:29284215
Life Sciences Inhibition of SRC/FAK cue: A novel pathway for the synergistic effect of rosuvastatin on the anti-cancer effect of dasatinib in hepatocellular carcinoma Pubmed: 30292831
Pediatric Research Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. Pubmed: 30464333
Journal of cellular physiology Targeting peptidylarginine deiminase reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis Pubmed: 30515825
Clinical and experimental pharmacology & physiology A prospective, randomized trial of intravenous hydroxocobalamin versus norepinephrine or saline for treatment of lipopolysaccharide‐induced hypotension in a swine … Pubmed: 30575111
Journal of Ocular Pharmacology and Therapeutics Evaluation of the Effects of Biohcly in an Model of Mechanical Wounds in the Rabbit Cornea Pubmed: 30888249
Advanced Healthcare Materials Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809
Cellular & Molecular Biology Letters MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1
ARCHIVES OF ORAL BIOLOGY Histopathological and Biochemical Evaluation of the effect of Paeoniflorin on the Periodontium During and After Periodontitis Formation in Rats Pubmed: 31005686
МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ
Mediators of Inflammation Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma
International Journal of Clinical and Experimental Medicine Expression of MMP-9 and TIMP-1 in rat models of pressure ulcer and their significance
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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MAA553Hu22 Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC; IP.
MAA553Hu21 Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC; IP.
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KSA553Hu01 ELISA Kit DIY Materials for Matrix Metalloproteinase 9 (MMP9) Main materials for "Do It (ELISA Kit) Yourself".