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Active Galectin 3 (GAL3)

LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

ACTIVITY TEST

Galectin 3 (GAL3) is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. The protein also has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. GAL3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. It also can agglutinate red blood. In this case, we choose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (HA) is as follows, two-fold dilute the recombinant rat GAL3 with 0.01M PBS (pH7.4), add 50μL a serial dilution of GAL3 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL3, replace with 50μL 0.01M PBS. Then add 50μL 1% RaE to each well and mixed gently. The plate is incubated for 1-2 hours at room temperature. The results are shown in Figure 1. The minimal effective concentration of GAL3 is 2.5μg/mL. (A) 1% RaE treated with 2.5μg/mL GAL3 for 2h; (B) Negative control without GAL3.
Figure. The hemagglutination activity of recombinant rat GAL3.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
Scientifi Reportcs Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices PubMed: 26582585
Clin Chim Acta Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population PubMed: 25578396
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4. pubmed:28431936
昆明医科大学学报 半乳糖凝集素3 在COPD 和肺癌中的表达及其意义. 2095610X
Archives of Medical Science Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department? publication:305625095
Biosensors & Bioelectronics A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod. pubmed:29096363
Scientific Reports The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction. pubmed:28827564
Clinica Chimica Acta Galectin-3 in patients with coronary heart disease and atrial fibrillation Pubmed:29287900
Advances in Experimental Medicine and Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922
american journal of physiology-heart and circulatory physiology Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus Pubmed: 32216615
Cardiology Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation Pubmed: 32516780
AUSTRALIAN DENTAL JOURNAL The effect of initial periodontal treatment on gingival crevicular fluid galectin©\3 levels in participants with periodontal disease 33378559
Cell Death & Disease Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis 33771973
Int J Clin Pract Serum Galectin©\3 Levels in Patients with Psoriasis 34137138
Phytomedicine Zerumbone, a humulane sesquiterpene from Syringa pinnatifolia, attenuates cardiac fibrosis by inhibiting of the TGF-β1/Smad signaling pathway after myocardial … Pubmed:35405613
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … Pubmed:35687771
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA303Ra01 Active Galectin 3 (GAL3) Cell culture; Activity Assays.
RPA303Ra01 Recombinant Galectin 3 (GAL3) Positive Control; Immunogen; SDS-PAGE; WB.
PAA303Ra01 Polyclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC; IP.
LAA303Ra81 FITC-Linked Polyclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC; IF.
LAA303Ra71 Biotin-Linked Polyclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC.
MAA303Ra24 Monoclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC; IP.
MAA303Ra23 Monoclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC; IP.
MAA303Ra21 Monoclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC; IP.
MAA303Ra22 Monoclonal Antibody to Galectin 3 (GAL3) WB; IHC; ICC; IP.
SEA303Ra ELISA Kit for Galectin 3 (GAL3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA303Ra Multiplex Assay Kit for Galectin 3 (GAL3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.