Active Elastase 2, Neutrophil (ELA2)

ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase

ACTIVITY TEST

Figure 1. The binding activity of ELA2 with COL17.
Elastase2 (ELA2), elastase 2 is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils and macrophages during inflammation, it destroys bacteria and host tissue. The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). Neutrophil elastase may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Besides, Collagen Type XVII (COL17) has been identified as an interactor of ELA2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COL17 and recombinant human ELA2. Briefly, ELA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to COL17-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ELA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ELA2 and COL17 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Mediators of Inflammation Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation Hindawi: 612848
Journal of Photochemistry and Photobiology B: Biology A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins Pubmed:25732262
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
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