Active Defensin Beta 2 (DEFb2)
B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
- Product No.APA072Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
200µg 1mg 5mg
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Figure. The binding activity of DEFb2 with KCNA3.
Defensin Beta 2 (DEFb2) known as skin-antimicrobial peptide 1 (SAP1) is a cysteine-rich cationic low molecular weight antimicrobial peptide. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. DEFb2 is produced by a number of epithelial cells and exhibits potent antimicrobial activity against Gram-negative bacteria and Candida, but not Gram-positive S. aureus. Besides, potassium voltage-gated channel, shaker-related subfamily, member 3 (KCNA3) has been identified as an interactor of DEFb2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DEFb2 and recombinant human KCNA3. Briefly, DEFb2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to KCNA3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DEFb2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DEFb2 and KCNA3 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Livestock Science||The effect of Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets ScienceDirect: S1871141311000126|
|Appl Environ Microbiol.||Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source|
|Plos Pathogens||HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis PubMed: PMC3715414|
|British Journal of Nutrition||Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699|
|University of Waikato||The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis Waikato:Source|
|Vaccine||On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment Pubmed:25444792|
|International Journal of Dairy Technology||Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111|
|Molecular Nutrition Food Research||Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948|
|Respiratory Research||Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner. pubmed:27655266|
|Key Engineering Materials||The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture. 16629795|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|APA072Hu01||Active Defensin Beta 2 (DEFb2)||Cell culture; Activity Assays.|
|RPA072Hu01||Recombinant Defensin Beta 2 (DEFb2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA072Hu01||Polyclonal Antibody to Defensin Beta 2 (DEFb2)||WB; ICC; IHC-P; IHC-F; ELISA.|
|LAA072Hu71||Biotin-Linked Polyclonal Antibody to Defensin Beta 2 (DEFb2)||WB; IHC; ICC.|
|MAA072Hu22||Monoclonal Antibody to Defensin Beta 2 (DEFb2)||WB; IHC; ICC; IP.|
|SEA072Hu||ELISA Kit for Defensin Beta 2 (DEFb2)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA072Hu01||ELISA Kit DIY Materials for Defensin Beta 2 (DEFb2)||Main materials for "Do It (ELISA Kit) Yourself".|