Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1
- Product No.APA040Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point5.1
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Figure. The chemotactic effect of CX3CL1 on THP-1 cells
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (15.625ng/mL, 31.25ng/mL, 62.5ng/mL and 125ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of CX3CL1 occurs at 15.625-125ng/mL.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 31.25ng/mL CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.
Figure. The chemotactic effect of CX3CL1 on THP-1 cells.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
|World Journal of Gastroenterology||Preliminary study correlating CX3CL1/CX3CR1 expression with gastric carcinoma and gastric carcinoma perineural invasion NCBI: PMC3989981|
|International Journal of Colorectal Disease||Downregulation of CX3CR1 ameliorates experimental colitis: evidence for CX3CL1-CX3CR1-mediated immune cell recruitment. pubmed:27942903|
|Frontiers in Cellular and Infection Microbiology||Reduced CX3CL1 Secretion Contributes to the Susceptibility of Oral Leukoplakia-Associated Fibroblasts to Candida albicans. pubmed:27891323|
|Blood Coagul Fibrinolysis||Low shear stress upregulates the expression of fractalkine through the activation of mitogen-activated protein kinases in endothelial cells Pubmed:29406386|
|Journal of Neuroinflammation||The prenatal challenge with lipopolysaccharide and polyinosinic: polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of … Pubmed: 32829711|
|Cells||Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200–CD200R and … Pubmed: 32664639|
|Journal of Neuroinflammation||CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats Pubmed: 32532282|
|JOURNAL OF PERIODONTAL RESEARCH||Potential biomarkers reflecting inflammation in patients with severe periodontitis: Fractalkine (CX3CL1) and its receptor (CX3CR1) 33641164|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|APA040Ra01||Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||Cell culture; Activity Assays.|
|RPA040Ra01||Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA040Ra01||Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||WB; IHC; ICC; IP.|
|LAA040Ra81||FITC-Linked Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||WB; IHC; ICC; IF.|
|LAA040Ra71||Biotin-Linked Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||WB; IHC; ICC.|
|MAA040Ra21||Monoclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||WB; IHC; ICC; IP.|
|SEA040Ra||ELISA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA040Ra||CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)||Chemiluminescent immunoassay for Antigen Detection.|