Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)

NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1

ACTIVITY TEST

Figure. The chemotactic effect of CX3CL1 on THP-1 cells
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (15.625ng/mL, 31.25ng/mL, 62.5ng/mL and 125ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of CX3CL1 occurs at 15.625-125ng/mL.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 31.25ng/mL CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.

Figure. The chemotactic effect of CX3CL1 on THP-1 cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
World Journal of Gastroenterology Preliminary study correlating CX3CL1/CX3CR1 expression with gastric carcinoma and gastric carcinoma perineural invasion NCBI: PMC3989981
International Journal of Colorectal Disease Downregulation of CX3CR1 ameliorates experimental colitis: evidence for CX3CL1-CX3CR1-mediated immune cell recruitment. pubmed:27942903
Frontiers in Cellular and Infection Microbiology Reduced CX3CL1 Secretion Contributes to the Susceptibility of Oral Leukoplakia-Associated Fibroblasts to Candida albicans. pubmed:27891323
Blood Coagul Fibrinolysis Low shear stress upregulates the expression of fractalkine through the activation of mitogen-activated protein kinases in endothelial cells Pubmed:29406386
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA040Ra01 Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) Cell culture; Activity Assays.
RPA040Ra01 Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA040Ra01 Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) WB; IHC; ICC; IP.
LAA040Ra81 FITC-Linked Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) WB; IHC; ICC; IF.
LAA040Ra71 Biotin-Linked Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) WB; IHC; ICC.
MAA040Ra21 Monoclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) WB; IHC; ICC; IP.
SEA040Ra ELISA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA040Ra CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) Chemiluminescent immunoassay for Antigen Detection.