Active Fibroblast Growth Factor 6 (FGF6)
HBGF6; HST2; HST2 Oncogene; Heparin Secretory-Transforming Protein 2; Heparin-Binding Growth Factor 6
- Product No.APA035Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 418
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For more details, please contact local distributors! US$ 6270
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Figure. The binding activity of FGF6 with FGFR1.
Fibroblast growth factor 6 (FGF6) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. It may also play a importent role in muscle regeneration or differentiation. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF6, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF6 and recombinant human FGFR1. Briefly, FGF6 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF6 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA035Hu01||Recombinant Fibroblast Growth Factor 6 (FGF6)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA035Hu01||Active Fibroblast Growth Factor 6 (FGF6)||Cell culture; Activity Assays.|
|PAA035Hu01||Polyclonal Antibody to Fibroblast Growth Factor 6 (FGF6)||WB; IHC; ICC; IP.|
|LAA035Hu81||FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 6 (FGF6)||WB; IHC; ICC; IF.|
|LAA035Hu71||Biotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 6 (FGF6)||WB; IHC; ICC.|
|MAA035Hu22||Monoclonal Antibody to Fibroblast Growth Factor 6 (FGF6)||WB; IHC; ICC; IP.|
|SEA035Hu||ELISA Kit for Fibroblast Growth Factor 6 (FGF6)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA035Hu||CLIA Kit for Fibroblast Growth Factor 6 (FGF6)||Chemiluminescent immunoassay for Antigen Detection.|