Active Brain Derived Neurotrophic Factor (BDNF)
- Product No.APA011Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.5
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Figure. The binding activity of BDNF with APP.
Brain-derived neurotrophic factor, also known as BDNF, is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse BDNF and recombinant mouse APP. Briefly, BDNF were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to APP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of BDNF and APP was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Journal of the Neurological Sciences||Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord ScienceDirect: S0022510X12001219|
|International Conference on Bioscience||Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059|
|Neuroscience Letters||Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788|
|BioMed Research International||Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622|
|Behavioural Brain Research||The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810|
|Neurochemical Research||Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord. Pubmed:26559686|
|Exp Ther Med.||Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|The International Journal af Applied Research in Veterinary Medicine||Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3|
|J Chem Neuroanat.||Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953|
|Int Immunopharmacol.||PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378|
|The Journal of Maternal-Fetal & Neonatal Medicine||Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|Bipolar Disorders||Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449|
|Elsevier||Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article|
|journal of allergy and clinical immunology||Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis pubmed:28412394|
|American Journal of Translational Research||A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979|
|European Journal of Pharmacology||Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA011Mu02||Recombinant Brain Derived Neurotrophic Factor (BDNF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA011Mu01||Recombinant Brain Derived Neurotrophic Factor (BDNF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA011Mu01||Active Brain Derived Neurotrophic Factor (BDNF)||Cell culture; Activity Assays.|
|PAA011Mu02||Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)||WB; IHC; ICC; IP.|
|PAA011Mu01||Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)||WB; IHC; ICC; IP.|
|SEA011Mu||ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA011Mu01||ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)||Main materials for "Do It (ELISA Kit) Yourself".|