ELISA Kit for Anti-Collagen Type II Antibody (Anti-COL2)

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-Collagen Type II Antibody (Anti-COL2).
No significant cross-reactivity or interference between Anti-Collagen Type II Antibody (Anti-COL2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Anti-Collagen Type II Antibody (Anti-COL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Collagen Type II Antibody (Anti-COL2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-93 84
EDTA plasma(n=5) 97-104 101
heparin plasma(n=5) 91-104 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Collagen Type II Antibody (Anti-COL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Collagen Type II Antibody (Anti-COL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Collagen Type II Antibody (Anti-COL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-95% 80-92% 98-105% 98-105%
EDTA plasma(n=5) 79-103% 98-105% 79-91% 91-99%
heparin plasma(n=5) 79-88% 78-99% 93-101% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
Spine (Phila Pa 1976). No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes. Pubmed:25856264
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Materials Science and Engineering: C Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition Pubmed:24433895
義守大學生物技術與化學工程研究所學位論文 可拋棄式退化性關節炎電化學式尿液免疫感測器之研究 Electrochemical Immunosensor Detection of Urinary Biomaker for Osteoarthritis Diagnosis Publication: U0074-2807201500521300
Biomed Rep Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs PubMed: 26623021
Macromolecular Bioscience Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063
中国眼耳鼻喉科杂志 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响 article:11593
Biomedical Materials An innovative co-axial system to electrospin in situ crosslinked gelatin nanofibers. Pubmed:26987305
Scientific Reports Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds pubmed:27821864
PLoS One. Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788
Polymers Microsphere-Based Hierarchically Juxtapositioned Biphasic Scaffolds Prepared from Poly(Lactic-co-Glycolic Acid) and Nanohydroxyapatite for Osteochondral Tissue Engineering 2073-4360:8
PLOS ONE Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders 10.1371:journal.pone.0187564
PLoS One Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders 10.1371:journal.pone.0172181
Platelets. Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate. pubmed:29090639
Molecular Medicine Reports Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits Pubmed:29257344
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
PAA572Hu01 Polyclonal Antibody to Collagen Type II (COL2) WB; IHC; ICC; IP.
SEA572Hu ELISA Kit for Collagen Type II (COL2) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA572Hu ELISA Kit for Anti-Collagen Type II Antibody (Anti-COL2) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA572Hu CLIA Kit for Collagen Type II (COL2) Chemiluminescent immunoassay for Antigen Detection.