Polyclonal Antibody to Histone H4 (H4)


The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.


Western blotting: 0.5-2µg/mL;1:100-400
Immunohistochemistry: 5-20µg/mL;1:10-40
Immunocytochemistry: 5-20µg/mL;1:10-40
Optimal working dilutions must be determined by end user.


Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.



Magazine Citations
PLoS One. N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice Pubmed:24816808
Crit Care. Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation Pubmed:25260379
Biomed Res Int A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649891
Journal of Viral Hepatitis Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation pubmed:27660136
Cell Death & Disease Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis Pubmed: 30778043
Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Rhesus monkey (Simian), Felis catus; Feline (Cat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA289Mi02 Recombinant Histone H4 (H4) Positive Control; Immunogen; SDS-PAGE; WB.
RPA289Mi01 Recombinant Histone H4 (H4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA289Mi02 Polyclonal Antibody to Histone H4 (H4) WB; IHC; ICC; IP.
PAA289Mi01 Polyclonal Antibody to Histone H4 (H4) WB; IHC; ICC; IP.
SEA289Mi ELISA Kit for Histone H4 (H4) Enzyme-linked immunosorbent assay for Antigen Detection.