Multiplex Assay Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay)

WNT-12; SHFM6

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 94
EDTA plasma(n=5) 78-92 85
heparin plasma(n=5) 91-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 87-104% 90-99% 88-95%
EDTA plasma(n=5) 94-102% 96-104% 92-99% 78-93%
heparin plasma(n=5) 91-99% 79-93% 89-101% 79-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:WNT10B) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Arthritis & Rheumatology CTLA-4Ig–Induced T Cell Anergy Promotes Wnt-10b Production and Bone Formation in a Mouse Model Onlinelibrary: art.38319
Annals of The New York Academy of Sciences CTLA‐4Ig (abatacept) balances bone anabolic effects of T cells and Wnt‐10b with antianabolic effects of osteoblastic sclerostin Pubmed:29500936
International Journal of Medical Sciences Hypoalbuminemia differently affects the serum bone turnover markers in hemodialysis patients Pubmed: 31839746
Food & Function Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the … Pubmed: 33140795
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPP553Hu01 Recombinant Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Positive Control; Immunogen; SDS-PAGE; WB.
PAP553Hu01 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) WB; IHC
MAP553Hu21 Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) WB; IHC; ICC; IP.
SEP553Hu ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Enzyme-linked immunosorbent assay for Antigen Detection.
SCP553Hu CLIA Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Chemiluminescent immunoassay for Antigen Detection.
LMP553Hu Multiplex Assay Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.