Multiplex Assay Kit for Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay)

SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 92-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 88-96% 93-105% 85-93%
EDTA plasma(n=5) 94-101% 79-103% 79-99% 97-105%
heparin plasma(n=5) 79-89% 85-101% 78-89% 78-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SEMA3A) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Arthritis Research & Therapy Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus Biomed: ar3881
journal of invastigative dermatology Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus PubMed: 26121211
Neurology Neurofilament light chain level is a weak risk factor for the development of MS. pubmed:27521440
Development miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neurons pubmed:29180574
A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells
Life Sciences Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. Pubmed: 32603817
ACS NANO Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling Pubmed: 32413258
PLoS One Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes 33905439
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPL917Mu01 Recombinant Semaphorin 3A (SEMA3A) Positive Control; Immunogen; SDS-PAGE; WB.
RPL917Mu02 Recombinant Semaphorin 3A (SEMA3A) Positive Control; Immunogen; SDS-PAGE; WB.
PAL917Mu02 Polyclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
PAL917Mu01 Polyclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
LAL917Mu71 Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC.
SEL917Mu ELISA Kit for Semaphorin 3A (SEMA3A) Enzyme-linked immunosorbent assay for Antigen Detection.
LML917Mu Multiplex Assay Kit for Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.