Multiplex Assay Kit for Translocator Protein (TSPO) ,etc. by FLIA (Flow Luminescence Immunoassay)

IBP; PKBS; PBR; pk18; mDRC; MBR; BZRP; DBI; PTBR; Mitochondrial benzodiazepine receptor; Peripheral-Type Benzodiazepine Receptor/Recognition Site

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Translocator Protein (TSPO) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Translocator Protein (TSPO) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocator Protein (TSPO) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocator Protein (TSPO) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:TSPO) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Disease Markers Plasma Translocator Protein Levels and Outcomes of Acute Ischemic Stroke: A Pilot Study Pubmed:30034558
NEUROLOGICAL RESEARCH Prognostic significance of serum translocator protein in patients with spontaneous intracerebral hematoma£º preliminary findings 33357157
Transl Vis Sci Technol Serum Iba-1, GLUT5, and TSPO in Patients With Diabetic Retinopathy: New Biomarkers for Early Retinal Neurovascular Alterations? A Pilot Study Pubmed:35285861
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
SEJ628Ra ELISA Kit for Translocator Protein (TSPO) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ628Ra Multiplex Assay Kit for Translocator Protein (TSPO) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.