Multiplex Assay Kit for Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay)

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-105 88
EDTA plasma(n=5) 84-91 88
heparin plasma(n=5) 84-93 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-95% 80-102% 89-96% 91-98%
EDTA plasma(n=5) 95-102% 98-105% 97-104% 86-98%
heparin plasma(n=5) 78-99% 79-101% 97-105% 80-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PDGFB) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
AAPS PharmSciTech Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B-and siPDGFR-β-Containing Chitosan Nanoplexes pubmed:27975193
Marmara Pharmaceutical Journal In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes 5000184338
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
Cell Biology International Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathway Pubmed: 31535749
Ann Agric Environ Med PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis Pubmed: 32208584
Journal of Tissue Engineering Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders 33868627
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
SEC921Bo ELISA Kit for Platelet Derived Growth Factor Subunit B (PDGFB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC921Bo Multiplex Assay Kit for Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.