Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-99 95
EDTA plasma(n=5) 91-101 96
heparin plasma(n=5) 84-102 94
sodium citrate plasma(n=5) 96-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 88-101% 82-105% 93-105%
EDTA plasma(n=5) 94-101% 98-105% 86-101% 94-105%
heparin plasma(n=5) 92-101% 97-105% 84-96% 98-105%
sodium citrate plasma(n=5) 88-102% 93-101% 98-105% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FGF21) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Lipids in Health and Disease Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs Lipidworld: source
Endocrinology Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source
Biochemical and Biophysical Research Communications Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH Pubmed:25449271
Free Radic Biol Med Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway PubMed: 25843656
Journal of diabetes and its complications Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484
American journal of the medical sciences Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus pubmed:29078839
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555
Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим …
Physiol Res Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. Pubmed: 32469231
Chinese Journal of Osteoporosis 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究.
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309
Nutrients The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
Medicine (Baltimore) Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort 34918690
Annals of Agricultural and Environmental Medicine Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074
Lab Invest Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury Pubmed:35488034
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPC918Po01 Recombinant Fibroblast Growth Factor 21 (FGF21) Positive Control; Immunogen; SDS-PAGE; WB.
PAC918Po01 Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
MAC918Po21 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
SEC918Po ELISA Kit for Fibroblast Growth Factor 21 (FGF21) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC918Po CLIA Kit for Fibroblast Growth Factor 21 (FGF21) Chemiluminescent immunoassay for Antigen Detection.
LMC918Po Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.