Multiplex Assay Kit for Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 91
EDTA plasma(n=5) 84-99 90
heparin plasma(n=5) 85-102 92
sodium citrate plasma(n=5) 78-99 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 95-103% 80-92% 89-96%
EDTA plasma(n=5) 91-104% 84-103% 79-104% 78-101%
heparin plasma(n=5) 80-91% 85-92% 80-89% 94-101%
sodium citrate plasma(n=5) 83-93% 87-101% 84-96% 83-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SALb) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Maternal-Fetal and Neonatal Medicine Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia Informa: 14767058.2012.660221
Peptides The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass PubMed: 22884920
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533
European Journal of Obstetrics & Gynecology and Reproductive Biology Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. PubMed: 23178004
Biotech Histochem. Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose. PubMed: 23244237
Gynecological Endocrinology, Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome. PubMed: 22827297
Peptides Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus ScienceDirect: S0196978113003100
Biotechnic &amp; Histochemistry Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Com:Source
Med Sci Monit. Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome Pubmed:Pmc4136931
Acta Physiologica Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats Pubmed:24304512
Pediatr Nephrol. Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? Pubmed:25245503
European Journal of Dermatology Serum salusin-α and salusin-β levels in patients with psoriasis PubMed: 26055619
J Investig Med Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease PubMed: 25730454
Genet Test Mol Biomarkers.  Serum Salusin-β Levels Are Correlated with Slow Coronary Flow. Pubmed:27227272
Sci Rep.  Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. pubmed:28230187
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148
Oxidative Medicine and Cellular Longevity Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma 10.1155:2017
International Journal Of Cardiology Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus Pubmed: 30581101
Multiple Sclerosis and Related Disorders Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis Pubmed: 30738876
cardiology in the young Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity Pubmed: 31486354
Redox Biology Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway Pubmed: 31884071
Journal of international medical research Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis Pubmed: 32054363
cardiovascular drugs and therapy A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats. Pubmed: 32458319
Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors
Mol Med Rep Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner 34296310
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CPC026Ra21 OVA Conjugated Salusin Beta (SALb) Immunogen; SDS-PAGE; WB.
CEC026Ra ELISA Kit for Salusin Beta (SALb) Enzyme-linked immunosorbent assay for Antigen Detection.
WEC026Ra Wide-range ELISA Kit for Salusin Beta (SALb) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC026Ra Multiplex Assay Kit for Salusin Beta (SALb) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC026Ra11 ELISA Kit DIY Materials for Salusin Beta (SALb) Main materials for "Do It (ELISA Kit) Yourself".