Multiplex Assay Kit for Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay)

ARIA; GGF; HGL; HRG; HRGA; NDF; SMDF; Acetylcholine receptor-inducing activity; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-101 86
EDTA plasma(n=5) 85-92 89
heparin plasma(n=5) 89-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 90-101% 90-104% 90-98%
EDTA plasma(n=5) 80-102% 88-95% 97-104% 80-96%
heparin plasma(n=5) 85-101% 81-103% 90-97% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NRG1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Neuroscience Letter Therapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndrom Cyagen: source
PLoS One. ADAM17 Silencing in Mouse Colon Carcinoma Cells: The Effect on Tumoricidal Cytokines and Angiogenesis PubMed: PMC3519469
The Journal of Clinical Investigation Cerebrospinal fluid–based kinetic biomarkers of axonal transport in monitoring neurodegeneration PubMed: PMC3428100
Physiol Behav Dysregulation of Neuregulin-1/ErbB signaling in the prefrontal cortex and hippocampus of rats exposed to chronic unpredictable mild stress PubMed: 26626816
J Toxicol Sci.  Effects of prolonged antipsychotic administration on neureCavia (Guinea pig )lin-1/ErbB signaling in rat prefrontal cortex and myocardium: implications for the therapeutic action and cardiac adverse effect. Pubmed:26961615
journal of cellular and molecular medicine Qiliqiangxin protects against anoxic injury in cardiac microvascular endothelial cells via NRG-1/ErbB-PI3K/Akt/mTOR pathway. pubmed:28271613
Transl Psychiatry. Ablation of neuropsin-neuregulin 1 signaling imbalances ErbB4 inhibitory networks and disrupts hippocampal gamma oscillation. pubmed:28267150
Human Molecular Genetics A proteasome-resistant fragment of NIK mediates oncogenic NF-κB signaling in schwannomas Pubmed: 30335132
Aging (Albany NY) Jinmaitong ameliorates diabetic peripheral neuropathy in streptozotocin-induced diabetic rats by modulating gut microbiota and neuregulin Pubmed: 32920546
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB866Mu01 Recombinant Neuregulin 1 (NRG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB866Mu02 Recombinant Neuregulin 1 (NRG1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB866Mu01 Polyclonal Antibody to Neuregulin 1 (NRG1) WB; IHC; ICC; IP.
PAB866Mu02 Polyclonal Antibody to Neuregulin 1 (NRG1) WB; IHC; ICC; IP.
SEB866Mu ELISA Kit for Neuregulin 1 (NRG1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB866Mu Multiplex Assay Kit for Neuregulin 1 (NRG1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.