Multiplex Assay Kit for Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay)

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SHH) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Annals of the Rheumatic Diseases Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB831Ra01 Recombinant Hedgehog Homolog, Sonic (SHH) Positive Control; Immunogen; SDS-PAGE; WB.
RPB831Ra02 Recombinant Hedgehog Homolog, Sonic (SHH) Positive Control; Immunogen; SDS-PAGE; WB.
PAB831Ra01 Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
PAB831Ra02 Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
LAB831Ra71 Biotin-Linked Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC.
MAB831Ra21 Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
SEB831Ra ELISA Kit for Hedgehog Homolog, Sonic (SHH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB831Ra Multiplex Assay Kit for Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB831Ra01 ELISA Kit DIY Materials for Hedgehog Homolog, Sonic (SHH) Main materials for "Do It (ELISA Kit) Yourself".