Multiplex Assay Kit for Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay)

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 89-97 93
heparin plasma(n=5) 92-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 85-104% 87-102% 94-103%
EDTA plasma(n=5) 85-103% 79-94% 99-105% 78-90%
heparin plasma(n=5) 84-98% 78-96% 83-95% 78-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FABP4) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Clinical and Molecular Hepatology Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH PubMed: 23091808
journal of Human Hypertension Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults PubMed: 26134622
genetics and molecular research Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice PubMed: 25867423
Food Chem Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes PubMed: 25952883
Placenta Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia Pubmed:26992681
Clin Chim Acta. Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. pubmed:28161274
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871
Nutrition & Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans Pubmed:29335416
American Journal of Cancer Research 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4 Pubmed: 31720079
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074
cells The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure Pubmed:35455943
Bone Research FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis Pubmed:35729106
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB693Ra01 Recombinant Fatty Acid Binding Protein 4 (FABP4) Positive Control; Immunogen; SDS-PAGE; WB.
PAB693Ra01 Polyclonal Antibody to Fatty Acid Binding Protein 4 (FABP4) WB; IHC; ICC; IP.
SEB693Ra ELISA Kit for Fatty Acid Binding Protein 4 (FABP4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB693Ra Multiplex Assay Kit for Fatty Acid Binding Protein 4 (FABP4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.