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Multiplex Assay Kit for Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay)
PST; HAST1/HAST2; ST1A3; STP1; TSPST1; Phenol Sulfotransferase; Aryl sulfotransferase 1; Phenol sulfotransferase 1; Thermostable phenol sulfotransferase
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB268Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 415
US$ 431
US$ 455
US$ 487
US$ 519
US$ 567
US$ 638
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 90-97 | 93 |
EDTA plasma(n=5) | 81-98 | 88 |
heparin plasma(n=5) | 80-99 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-101% | 93-102% | 78-95% | 88-102% |
EDTA plasma(n=5) | 92-105% | 87-98% | 82-89% | 83-94% |
heparin plasma(n=5) | 78-101% | 94-104% | 84-101% | 80-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:SULT1A1) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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INCREMENT SERVICES
Magazine | Citations |
Reproductive Toxicology | Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary. PubMed: 22750137 |
The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 PubMed: 25733458 |
Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590 |
Molecular Nutrition & Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590 |
Redox Biology | STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. pubmed:29078168 |
Available at SSRN 3234834 | Human Plasma Protein (s) Based Conceptual Diagnostic Tool for Altitude-Acclimation Assessment |
Life sciences | Reverse translating SULT1A1, a potential biomarker in roentgenographically tested rat model of rapid HAPE induction Pubmed: 31100325 |
Functional & Integrative Genomics | Plasma protein (s)–based conceptual diagnostic tool for assessing high-altitude acclimation in humans Pubmed: 31444657 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB268Hu01 | Recombinant Sulfotransferase 1A1 (SULT1A1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB268Hu01 | Polyclonal Antibody to Sulfotransferase 1A1 (SULT1A1) | WB; IHC; ICC; IP. |
MAB268Hu22 | Monoclonal Antibody to Sulfotransferase 1A1 (SULT1A1) | WB; IHC; ICC; IP. |
SEB268Hu | ELISA Kit for Sulfotransferase 1A1 (SULT1A1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB268Hu | Multiplex Assay Kit for Sulfotransferase 1A1 (SULT1A1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |