Multiplex Assay Kit for Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay)

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-103 97
EDTA plasma(n=5) 87-96 92
heparin plasma(n=5) 78-90 85
sodium citrate plasma(n=5) 79-93 80

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-105% 82-95% 78-95% 93-102%
EDTA plasma(n=5) 90-98% 98-105% 89-103% 97-104%
heparin plasma(n=5) 99-105% 88-97% 80-104% 96-103%
sodium citrate plasma(n=5) 88-95% 80-95% 88-97% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CD73) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Autoimmunity The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation Pubmed:25640206
Talanta Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … 10.1016:j.talanta.2018.01.044
Cancer Biomarkers Expression and clinical significance of serum NT5E protein in patients with colorectal cancer Pubmed: 30932882
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB250Hu01 Active Cluster Of Differentiation 73 (CD73) Cell culture; Activity Assays.
RPB250Hu01 Recombinant Cluster Of Differentiation 73 (CD73) Positive Control; Immunogen; SDS-PAGE; WB.
PAB250Hu01 Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
FAB250Hu01 Anti-Cluster Of Differentiation 73 (CD73) Polyclonal Antibody Flow cytometry.
LAB250Hu81 FITC-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IF.
LAB250Hu71 Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC.
FAB250Hu41 PE-Linked Anti-Cluster Of Differentiation 73 (CD73) Polyclonal Antibody Flow cytometry.
FAB250Hu51 APC-Linked Anti-Cluster Of Differentiation 73 (CD73) Polyclonal Antibody Flow cytometry.
FAB250Hu81 FITC-Linked Anti-Cluster Of Differentiation 73 (CD73) Polyclonal Antibody Flow cytometry.
RAB250Hu21 Recombinant Antibody to Cluster Of Differentiation 73 (CD73) WB; IF; ICC; IHC; IP; FCM.
MAB250Hu21 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
MAB250Hu24 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
MAB250Hu25 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
MAB250Hu22 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
MAB250Hu27 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
MAB250Hu29 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
MAB250Hu23 Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
SEB250Hu ELISA Kit for Cluster Of Differentiation 73 (CD73) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB250Hu Multiplex Assay Kit for Cluster Of Differentiation 73 (CD73) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB250Hu01 ELISA Kit DIY Materials for Cluster Of Differentiation 73 (CD73) Main materials for "Do It(ELISA Kit) Yourself"