Multiplex Assay Kit for Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay)

HL1; INTL; ITLN; LFR; HIntL; Omentin; Intelectin 1,Galactofuranose Binding; Intestinal Lactoferrin Receptor;Endothelial lectin HL-1

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-105 97
EDTA plasma(n=5) 85-102 89
heparin plasma(n=5) 88-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 90-98% 79-94% 79-101%
EDTA plasma(n=5) 92-105% 86-104% 78-101% 93-101%
heparin plasma(n=5) 89-103% 97-105% 89-97% 89-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ITLN1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Plos one Cardioprotective Properties of Omentin-1 in Type 2 Diabetes: Evidence from Clinical and In Vitro Studies PubMed: PMC3612072
Rev Port Cardiol. Serum visfatin and omentin levels in slow coronary flow Pubmed:25481776
Thieme Evaluation of the Relation Between Omentin-1 and Vitamin D in Postmenopausal Women With or Without Osteoporosis DOI: 10.1055/s-0043-120110
Archives of Gynecology and Obstetrics Maternal and neonatal omentin-1 levels in gestational diabetes Pubmed:29335783
Journal of Cardiovascular Translational Research Long-Term Weight Gain Associated With High Omentin Levels at Hospital Discharge Improves Prognosis of Patients Following Acute Heart Failure Pubmed: 30353296
Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar Pubmed: 30232744
Iranian Journal of Pediatrics The Comparison of Cord Blood Omentin-1 Values of Newborns in the Different Birth Weights According to Gestational Age
Mucosal Immunology ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism Pubmed: 31278375
Nature Communications ITLN1 modulates invasive potential and metabolic reprogramming of ovarian cancer cells in omental microenvironment Pubmed: 32669559
A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME)
cells The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure Pubmed:35455943
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA933Hu01 Recombinant Intelectin 1 (ITLN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA933Hu01 Polyclonal Antibody to Intelectin 1 (ITLN1) WB; IHC
MAA933Hu22 Monoclonal Antibody to Intelectin 1 (ITLN1) WB; IHC; ICC; IP.
SEA933Hu ELISA Kit for Intelectin 1 (ITLN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA933Hu Multiplex Assay Kit for Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.