Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay)

SCGB1A1; CC10; CCSP; UGB; UG; Uteroglobin; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-104 89
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 86-101 89
sodium citrate plasma(n=5) 82-96 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 83-97% 81-94% 82-92%
EDTA plasma(n=5) 85-101% 96-104% 97-105% 88-97%
heparin plasma(n=5) 78-96% 88-105% 78-101% 94-103%
sodium citrate plasma(n=5) 86-98% 91-99% 80-91% 78-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CC16) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Life Science Journal Pulmonary functions and blood biochemical markers for workers with and without coal worker pneumoconiosis Sciencepub: Source
Current Therapeutic Research Analysis of the Protective Biochemical and Pathologic Effects of Aminoguanidine on an Experimental Aspiration Pneumonitis Model Induced by Bile Acids PubMed: S0011393X12000562
J Thorac Dis. Effects of leflunomide on inflamation and fibrosis in bleomycine induced pulmonary fibrosis in wistar albino rats Pubmed: 24255778
J Mol Histol. The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats Pubmed: 24122261
Hum Exp Toxicol January The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acids Pubmed: 23023024
Journal of Thoracic Disease The evaluation of different treatment protocols for trauma-induced lung injury in rats Pubmed: 24605218
PLOS ONE Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking Pubmed:25036363
PLoS One Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis … Pubmed:30024955
Bioscience Reports N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. Doi: 10.1042/BSR20180858
biorxiv Mechanical activation of TRPV4 channels controls albumin reabsorption by proximal tubule cells.
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
Effects of mammary nutrient demand on glucose kinetics of dairy cows in early lactation
Biotech Histochem Role of bone marrow-derived mesenchymal stem cells in alleviating pulmonary epithelium damage and extracellular matrix remodeling in a rat model of lung fibrosis … Pubmed: 32909452
OBESITY SURGERY The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized … Pubmed: 32535786
Respir Res Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital … Pubmed:35189887
Drug Des Devel Ther Lung-Protective Effects of Lidocaine Infusion on Patients with Intermediate/High Risk of Postoperative Pulmonary Complications: A Double-Blind Randomized … Pubmed:35422611
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