Multiplex Assay Kit for Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay)

CCK-PZ

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-102 97
EDTA plasma(n=5) 97-104 101
heparin plasma(n=5) 79-101 96
sodium citrate plasma(n=5) 78-102 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 92-105% 92-101% 91-98%
EDTA plasma(n=5) 80-102% 96-104% 95-105% 98-105%
heparin plasma(n=5) 97-105% 78-103% 97-105% 87-94%
sodium citrate plasma(n=5) 92-101% 89-101% 85-99% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CCK) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Journal of Obstetrics and Gynaecology Research? Disturbed release of cholecystokinin in pregnant women with hyperemesis gravidarum Pubmed:25331205
World J Gastroenterol Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732
Journal of Ethnopharmacology Luhong formula inhibits myocardial fibrosis in a paracrine manner by activating thegp130/JAK2/STAT3 pathway in cardiomyocytes. pubmed:28115285
Cellular physiology and biochemistry Cholecystokinin Expression in the Development of Postinfarction Heart Failure. pubmed:29130944
JOURNAL OF PHARMACEUTICAL SCIENCES Assessment of the role of cholecystokinin in hyperemesis gravidarum and correlation with its severity :
Journal of Dairy Science Effects of dietary leucine and phenylalanine on pancreas development, enzyme activity, and relative gene expression in milk-fed Holstein dairy calves Pubmed:29477524
Food and Nutrition Research Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet Pubmed:29599686
International Heart Journal The Relationship of Appetite-Regulating Hormones in the Development of Cardiac Cachexia Pubmed: 30799377
Chinese Medicine Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model Pubmed: 33014123
foods Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … 34828792
npj Biofilms and Microbiomes The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849
Food Funct Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC … Pubmed:35587126
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
CEA802Bo ELISA Kit for Cholecystokinin (CCK) Enzyme-linked immunosorbent assay for Antigen Detection.
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