Multiplex Assay Kit for Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay)

HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 79-90 78

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kidney Injury Molecule 1 (Kim1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 84-98% 80-89% 83-90%
EDTA plasma(n=5) 96-103% 85-96% 85-99% 80-92%
heparin plasma(n=5) 89-96% 92-105% 83-95% 78-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Kim1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Clinical Toxicology Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication PubMed: 19827907
Pediatric Nephrology KIM-1 and NGAL: new markers of obstructive nephropathy SpringerLink: v461592787w5488x
Pediatric Research Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants LWW: 10000
Archives of Medical Science Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate AMS: source
Nephrology Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content PubMed: 23046363
Nanotoxicology. Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats. PubMed: 23272807
Toxicological Science A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent Models PubMed: PMC3498743
Disease Markers Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment Hindawi: 369784
Journal of Biomarkers Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model Hindawi: 413853
Advances in Life Science and Technology Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury. Iiste: Source
Nephrology Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant Pubmed: 24325180
Nephrology Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content. Pubmed: 23046363
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829
Diabetes &amp; Vascular Disease Research Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats Sagepub:Source
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source
Nephrology (Carlton). Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipients Pubmed:24666430
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434
BMC Nephrology SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial Pubmed:24996842
Pediatric Nephrology The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers Pubmed:25127917
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Transworld Medical Journal Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injury Tmjournals:Source
Int J Clin Exp Pathol Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis PubMed: 26191125
Int J Clin Exp Pathol Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice PubMed: 26045773
Mol Med Rep Vorinostat protects against calcium oxalate-induced kidney injury in mice PubMed: 26095064
Pediatr Nephrol Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABP PubMed: 26324091
Journal of Pediatric Urology Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosis PubMed: 26096437
Am J Physiol Renal Physiol Intrarenal Renin-Angiotensin System Mediates Fatty Acid-Induced ER Stress in the Kidney PubMed: 26672616
Mol Med Rep. Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities. Pubmed:27082705
Nephrol. Dial. Transplant. Preconditioned suppression of prolyl-hydroxylases attenuates renal injury but increases mortality in septic murine models Pubmed:26908768
J Clin Lab Anal.  Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus Pubmed:27132791
Med Sci Monit. Protective Effect of Dihydromyricetin Against Lipopolysaccharide-Induced Acute Kidney Injury in a Rat Model pmc:PMC4754091
Diabetes. Deficiency in Apoptosis-Inducing Factor Recapitulates Chronic Kidney Disease via Aberrant Mitochondrial Homeostasis Pubmed:26822084
Sci Rep. Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db mice Pubmed:27226136
Journal of Applied Life Sciences International Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats Barakat812016JALSI28698.pdf
Oxidative Medicine and Cellular Longevity Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model. pubmed:28053694
Molecular Medicine Reports Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via itsantioxidant, anti‑inflammatory, and anti‑apoptotic activities. pubmed:27082705
Journal of Agricultural and Food Chemistry Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice pubmed:29254330
Clin Exp Pharmacol Physiol. Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. pubmed:29027259
Nephron - Physiology Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise 10.1159/000481179
Cellular Physiology and Biochemistry Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … Pubmed:29550811
Frontiers in Pharmacology Exploring the therapeutic mechanism of Desmodium styracifolium on oxalate crystal-induced kidney injuries using comprehensive approaches based on … Pubmed:29950996
Molecules Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy Pubmed:29642511
American Journal of Physiology-Regulatory Integrative and Comparative Physiology Modeling heart failure risk in diabetes and kidney disease: limitations and potential applications of transverse aortic constriction in high-fat-fed mice Pubmed:29443547
Biological Trace Element Research Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats Pubmed: 30251228
Cancer Research and Treatment A Phage Display-Identified Peptide Selectively Binds to Kidney Injury Molecule-1(KIM-1) and Detects KIM-1-Overexpressing Tumors in vivo
Archives of Medical Science Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, N-acetyl-β-D-glucosaminidase levels and mortality risk in septic patients with … Pubmed: 30393493
Biological & Pharmaceutical Bulletin UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury Pubmed: 30381664
Kidney and Blood Pressure Research The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy Pubmed: 30504713
Clinica Chimica Acta Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional study Pubmed: 30684459
BMC Complementary and Alternative Medicine Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine Pubmed: 30871515
2.583 Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors Doi: BioMed Research International
Naunyn-Schmiedeberg's archives of pharmacology Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade Pubmed: 31197431
Egyptian Journal of Zoology Effect of Cucumis melo var. flexuosus leaves extract on renal oxidative injury and inflammation in diabetic male albino rats
Environment International Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures Pubmed: 31639605
Bioscience Reports page Metabolomics study of fasudil on cisplatin-induced kidney injury Pubmed: 31670380
life sciences MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injury Pubmed: 31790686
Arch Physiol Biochem Protective effect of saxagliptin against renal ischaemia reperfusion injury in rats Pubmed: 31979992
clinical science Delineating a role for the Mitochondrial Permeability Transition Pore in Diabetic Kidney Disease by targeting Cyclophilin D Pubmed: 31943002
LIFE SCIENCES Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway Pubmed: 32798557
INTERNATIONAL UROLOGY AND NEPHROLOGY Serum NGAL, KIM-1, IL-18, L-FABP: new biomarkers in the diagnostics of acute kidney injury (AKI) following invasive cardiology procedures Pubmed: 32557377
Journal of Pediatric Urology Can Serum Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Help in Decision Making for Surgery in Antenatally Dedected … Pubmed: 33139211
Ratlarda sodyum florid ile oluşturulan toksikasyonda Karvakol'ün böbrekler üzerine etkisi
Rationale and design of the intervention in PHOspholamban RElated CArdiomyopathy STudy (i-PHORECAST)
Pediatr Int Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers 33423326
J Int Med Res Value of urinary kidney injury molecule-1 levels in predicting acute kidney injury in very low birth weight preterm infants 33372811
Biomed Res Int Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury 33604384
Nutrients Targeting Methylglyoxal in Diabetic Kidney Disease Using the Mitochondria-Targeted Compound MitoGamide 33922959
J Am Soc Nephrol Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI 34127536
Heliyon Anti-oxidant impact of Lisinopril and Enalapril against acute kidney injury induced by doxorubicin in male Wistar rats: involvement of kidney injury molecule-1 33506137
Journal of Pharmacological Sciences Pentoxifylline treatment alleviates kidney ischemia/reperfusion injury: Novel involvement of galectin-3 and ASK-1/JNK & ERK1/2/NF-¦ÊB/HMGB-1 trajectories 34030796
Int Urol Nephrol Could urinary kidney injury molecule-1 be a good marker in subclinical acute kidney injury in mild to moderate COVID-19 infection? 34213713
Intensive Care Medicine Experimental Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental … 34169407
iscience Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure 34820603
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SEA785Po ELISA Kit for Kidney Injury Molecule 1 (Kim1) Enzyme-linked immunosorbent assay for Antigen Detection.
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