Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay)

CTX-II; CTX2

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-102 96
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 94-103 99
sodium citrate plasma(n=5) 89-96 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-105% 90-97% 98-105% 95-102%
EDTA plasma(n=5) 81-103% 79-90% 83-95% 98-105%
heparin plasma(n=5) 91-104% 83-93% 80-94% 88-101%
sodium citrate plasma(n=5) 84-99% 96-104% 94-101% 79-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CTXII) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Cartilage Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis A Pilot Study 1947603516675915.abstract
BioMed Research International Decreased Plasma COMP and Increased Plasma CTX-II Levels in a Chinese Pseudoachondroplasia Family with Novel COMP Mutation pubmed:29104872
In Vivo Osteochondral Repair Using Porous Three-dimensional Nanocomposite Scaffolds in a Rabbit Model pubmed:28882956
Aging Cell Transthyretin deposition promotes progression of osteoarthritis pubmed:28941045
Molecular Medicine Reports Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate‑induced osteoarthritis rats pubmed:29067461
Experimental and Clinical Sciences International online journal for advances in science Schisandrae Fructus ethanol extract ameliorates inflammatory responses and articular cartilage damage in monosodium iodoacetate-induced osteoarthritis in rats. pubmed:28507472
Advances in Clinical and Experimental Medicine Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with … 68362.pdf
Journal of Orthopaedic Surgery and Research The levels of urine CTX-II, C2C, and PYD in children patients with Kashin-Beck disease in Qinghai Province of China Pubmed: 30635000
Nutrients The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity Pubmed: 31311115
International journal of molecular sciences Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients Pubmed: 31717649
Applied Physiology Nutrition and Metabolism Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial Pubmed: 31990581
heliyon Oral intake of Streptococcus thermophilus improves knee osteoarthritis degeneration: A randomized, double-blind, placebo-controlled clinical study
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