Multiplex Assay Kit for Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay)

FV; FVL; PCCF; Proaccelerin; Labile Factor; Factor V Leiden; Accelerator Globulin; Activated protein C cofactor; Proaccelerin, labile factor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-102 98
EDTA plasma(n=5) 96-105 99
heparin plasma(n=5) 87-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 95-105% 78-96% 81-99%
EDTA plasma(n=5) 80-101% 91-105% 97-104% 92-101%
heparin plasma(n=5) 82-101% 80-101% 94-102% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:F5) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biochem Biophys Res Commun Thrombin generation in abdominal sepsis is Rho-kinase-dependent PubMed: 25813486
Cell Transplantation Evidence of a Pivotal Role for the Distal Part of the Complement Cascade in the Diurnal Release of Hematopoietic Stem Cells Into Peripheral Blood Pubmed:26087465
Cardiovascular Research Loss of accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity Pubmed:29444200
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA675Mu02 Recombinant Coagulation Factor V (F5) Positive Control; Immunogen; SDS-PAGE; WB.
RPA675Mu03 Recombinant Coagulation Factor V (F5) Positive Control; Immunogen; SDS-PAGE; WB.
RPA675Mu01 Recombinant Coagulation Factor V (F5) Positive Control; Immunogen; SDS-PAGE; WB.
PAA675Mu01 Polyclonal Antibody to Coagulation Factor V (F5) WB; IHC; ICC; IP.
PAA675Mu02 Polyclonal Antibody to Coagulation Factor V (F5) WB; IHC; ICC; IP.
PAA675Mu03 Polyclonal Antibody to Coagulation Factor V (F5) WB; IHC; ICC; IP.
SEA675Mu ELISA Kit for Coagulation Factor V (F5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA675Mu Multiplex Assay Kit for Coagulation Factor V (F5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA675Mu01 ELISA Kit DIY Materials for Coagulation Factor V (F5) Main materials for "Do It(ELISA Kit) Yourself"