Multiplex Assay Kit for Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay)

Major synaptic vesicle protein p38

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-103 97
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 89-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 95-104% 83-97% 97-105%
EDTA plasma(n=5) 86-93% 88-102% 89-97% 78-88%
heparin plasma(n=5) 93-101% 94-101% 79-99% 80-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SYP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuropsychopharmacology Increased Amyloid-β Peptide-Induced Memory Deficits in Phospholipid Transfer Protein (PLTP) Gene Knockout Mice Nature: Source
United States Application US20140378440 BACLOFEN AND ACAMPROSATE BASED THERAPY OF NEUROLOGICAL DISORDERS Read more: http://www.faqs.org/patents/app/20130090307#ixzz3VGRXUqW9 Faqs:Source
Scientific Reports In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Disease pubmed:27609215
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease
University of British Columbia Investigating the roles of SRRM4 in contribution to neuroendocrine prostate cancer progression
pharmacological research Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors Pubmed: 31048034
Nutrients Fermented Goat Milk Consumption Enhances Brain Molecular Functions during Iron Deficiency Anemia Recovery Pubmed: 31591353
ACS Applied Materials & Interfaces Tunable Substrate Functionalities Direct Stem Cell Fate toward Electrophysiologically Distinguishable Neuron-like and Glial-like Cells 33356098
JOURNAL OF MOLECULAR NEUROSCIENCE Enriched Environment Minimizes Anxiety/Depressive-Like Behavior in Rats Exposed to Immobilization Stress and Augments Hippocampal Neurogenesis (In Vitro) 33492617
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA425Mu01 Recombinant Synaptophysin (SYP) Positive Control; Immunogen; SDS-PAGE; WB.
RPA425Mu02 Recombinant Synaptophysin (SYP) Positive Control; Immunogen; SDS-PAGE; WB.
PAA425Mu02 Polyclonal Antibody to Synaptophysin (SYP) WB; IHC; ICC; IP.
PAA425Mu01 Polyclonal Antibody to Synaptophysin (SYP) WB; IHC; ICC; IP.
SEA425Mu ELISA Kit for Synaptophysin (SYP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA425Mu ELISA Kit for Synaptophysin (SYP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA425Mu Multiplex Assay Kit for Synaptophysin (SYP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.