Multiplex Assay Kit for Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay)

MME; HME; ME; Macrophage Metalloelastase; Macrophage Elastase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 95
EDTA plasma(n=5) 92-104 97
heparin plasma(n=5) 87-103 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 83-105% 94-102% 87-98%
EDTA plasma(n=5) 79-103% 90-103% 99-105% 88-98%
heparin plasma(n=5) 96-105% 80-101% 86-103% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:MMP12) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
International journal of colorectal disease Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair Pubmed: 23619615
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
American Journal of Physiology - Lung Cellular and Molecular Physiology Inspiratory resistive breathing induces MMP-9 and MMP-12 expression in the lung Pubmed:25595645
Photodermatol Photoimmunol Photomed. Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice pubmed:27995657
Яковлева Наталья Юрьевна
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
Nat Commun Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice Pubmed:35031603
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA402Mu01 Recombinant Matrix Metalloproteinase 12 (MMP12) Positive Control; Immunogen; SDS-PAGE; WB.
RPA402Mu02 Recombinant Matrix Metalloproteinase 12 (MMP12) Positive Control; Immunogen; SDS-PAGE; WB.
PAA402Mu02 Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12) WB; IHC; ICC; IP.
PAA402Mu01 Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12) WB; IHC; ICC; IP.
LAA402Mu71 Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12) WB; IHC; ICC.
LAA402Mu81 FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12) WB; IHC; ICC; IF.
SEA402Mu ELISA Kit for Matrix Metalloproteinase 12 (MMP12) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA402Mu Multiplex Assay Kit for Matrix Metalloproteinase 12 (MMP12) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.