Multiplex Assay Kit for Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay)

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 94-101 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stromal Cell Derived Factor 1 (SDF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 86-94% 96-103% 97-105%
EDTA plasma(n=5) 85-96% 98-105% 94-103% 91-98%
heparin plasma(n=5) 78-96% 84-99% 98-105% 95-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SDF1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Critical Care Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD) Goescholar: source
22 The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels ScienceDirect: S0142961209012654
22 The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms ScienceDirect: S0142961211011422
Phytomedicine Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model ScienceDirect: S0944711310003260
Graefe's Archive for Clinical and Experimental Ophthalmology Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells PubMed: 21655971
Molecular Therapy Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia PubMed: 21224834
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
International Journal of Nephrology Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis PubMed: 22792461
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
Experimental Eye Research Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells Pubmed: 24780853
Atherosclerosis The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial Pubmed:24530788
International Journal of Cardiology Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men Pubmed:24286591
Tissue Engineering Part A Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095
Pharmacol Rep.? Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression Pubmed:25150002
Journal of Stroke and Cerebrovascular Diseases Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients Pubmed:25444027
Pharmacological Reports The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders Pubmed:25560580
In Vitro Cellular &amp; Developmental Biology - Animal Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells Pubmed:25636237
Pharmacological Reports Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder PubMed: 26481549
Biochem Biophys Res Commun Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats PubMed: 26392311
Am J Transl Res Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666
Medicine (Baltimore) A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632727
Pharmacological Reports A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity Pubmed:27208878
Frontiers in Psychiatry Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. pubmed:28149283
European Journal of Applied Physiology High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia. pubmed:27761657
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs pubmed:29233725
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
BioMed Research International. c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer pubmed:28573141
Scientific Reports Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. pubmed:28842629
Biomaterials Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. pubmed:29017079
Biomaterials Science The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration Pubmed:29850672
International Journal of Molecular Medicine Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expression Pubmed:29620151
Cardiovascular Drugs and Therapy β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats Pubmed:29752864
Cytotherapy Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation Doi: 10.1016/j.jcyt.2018.07.007
ACS Applied Materials & Interfaces Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration Pubmed: 30777748
World Journal of Stem Cells Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells Pubmed: 30949297
Stem Cells International Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells Pubmed: 31827537
Stem Cell Res Ther Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus Pubmed: 32894200
Journal of tissue engineering and regenerative medicine Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4 Pubmed: 32633015
Acta Biomater Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia 33878473
pharmaceuticals bFGF and SDF-1¦Á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts 33800631
Microb Biotechnol Combination of an engineered Lactococcus lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice 34310856
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