Multiplex Assay Kit for Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay)

CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-103 95
EDTA plasma(n=5) 95-103 98
heparin plasma(n=5) 93-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 94-103% 78-96% 79-91%
EDTA plasma(n=5) 91-99% 89-101% 80-93% 97-104%
heparin plasma(n=5) 79-103% 83-94% 80-103% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:IL10) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Gastroprotective effect of Lycium barbarum polysaccharides and C-phyocyanin in rats with ethanol-induced gastric ulcer Pubmed: 33058973
JOURNAL OF GENE MEDICINE Rabbit umbilical cord mesenchymal stem cells: A New Option for tissue engineering Pubmed: 33047422
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535
Heliyon Effect of procyanidins on lipid metabolism and inflammation in rats exposed to alcohol and iron Pubmed: 32964156
Int J Neurosci Downregulation of ROCK2 attenuates alcohol-induced inflammation and oxidative stress in astrocytes Pubmed: 32942936
THERIOGENOLOGY Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion Pubmed: 32810792
Theriogenology A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows Pubmed: 32810791
JOURNAL OF MOLECULAR NEUROSCIENCE Different Activation of IL-10 in the Hippocampus and Prefrontal Cortex During Neurodegeneration Caused by Trimethyltin Chloride Pubmed: 32803646
BRAIN BEHAVIOR AND IMMUNITY Active smoking, sleep quality and cerebrospinal fluid biomarkers of neuroinflammation Pubmed: 32717405
Bioengineered Down-regulation of ROCK2 alleviates ethanol-induced cerebral nerve injury partly by the suppression of the NF-κB signaling pathway Pubmed: 32684089
EXPERIMENTAL DERMATOLOGY Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteins Pubmed: 32682346
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Pubmed: 32680553
Life Sci Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study Pubmed: 32652140
Front Pharmacol Cyclic Peptide Extracts Derived From Pseudostellaria heterophylla Ameliorates COPD via Regulation of the TLR4/MyD88 Pathway Proteins Pubmed: 32581806
BIOTECHNOLOGY LETTERS Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway Pubmed: 32567013
INTERNATIONAL IMMUNOPHARMACOLOGY A potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol … Pubmed: 32559721
Int J Mol Sci Effects of PDE3 Inhibitor Olprinone on the Respiratory Parameters, Inflammation, and Apoptosis in an Experimental Model of Acute Respiratory Distress Syndrome Pubmed: 32403267
BRAIN BEHAVIOR AND IMMUNITY USP8 protects against lipopolysaccharide-induced cognitive and motor deficits by modulating microglia phenotypes through TLR4/MyD88/NF-κB signaling pathway in … Pubmed: 32335193
JAMA Surgery Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment Pubmed: 28593326
BIOMEDICINE & PHARMACOTHERAPY Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats Pubmed: 33152939
Frontiers in Pharmacology M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice Pubmed: 33041798
κ‑opioid receptor agonists may alleviate intestinal damage in cardiopulmonary bypass rats by inhibiting the NF‑κB/HIF‑1α pathway Pubmed: 32509012
Molecular Medicine Reports Placenta‑derived mesenchymal stem cells ameliorate lipopolysaccharide‑induced inflammation in RAW264. 7 cells and acute lung injury in rats Pubmed: 32626979
Food & Function Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …
Nanoscale Research Letters Protective Effects of Carbon Dots Derived from Phellodendri Chinensis Cortex Carbonisata against Deinagkistrodon acutus Venom-Induced Acute Kidney …
Journal of Biochemical and Molecular Toxicology Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular …
Journal of Functional Foods Wine pomace product modulates oxidative stress and microbiota in obesity high-fat diet-fed rats
Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra
Applied materials today Tanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarization
letter in applied nanobioscience Phytochemical and Toxicological Effect of Ethanol Extract of Heliotropium Indicum on Liver of Male Albino Rats
biosciences biotechnology research asia Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.
Phytomedicine Menthacarin attenuates experimental colitis
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Immunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation study
Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness
Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis
Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokine
EC NEUROLOGY Malted Barley Ameliorated Spinal Cord Injuries of Offspring of Hypercholesterolemic Mother Rats
Journal of Burn Care & Research Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats 33313794
Stem Cell Reviews and Reports Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care 33469783
Antioxidants (Basel) Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model 33562069
european journal of clinical nutrition? Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury 33514869
Drug Des Devel Ther Convallatoxin Promotes M2 Macrophage Polarization to Attenuate Atherosclerosis Through PPAR¦Ã-Integrin ¦Áv¦Â5 Signaling Pathway 33654384
Biomed Res In Antiangiogenic Effect of Platelet P2Y12 Inhibitor in Ischemia-Induced Angiogenesis in Mice Hindlimb 33898623
BIOMATERIALS Aligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regeneration 33813259
BEHAVIOURAL BRAIN RESEARCH Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis 34000339
Life Sci Asiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-¦ÊB p65 mediated modulation of microglia polarization 33965378
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
Drug Design Development and Therapy Effects of Systemic Lidocaine on Postoperative Recovery Quality and Immune Function in Patients Undergoing Laparoscopic Radical Gastrectomy 33976537
Life Sciences p-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosis 33915129
Brain Res Bull Melatonin ameliorates anxiety-like behaviors induced by sleep deprivation in mice: role of oxidative stress, neuroinflammation, autophagy and apoptosis 34144202
IUBMB Life HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1 34117711
Anal Cell Pathol Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 34123712
Pediatr Res Hypothermia is not therapeutic in a neonatal piglet model of inflammation-sensitized hypoxia¨Cischemia 34050269
Stem Cells International The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­ 33381190
BioMed Research International Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡­ 33376718
cells The Effect of the Clenbuterol¡ª¦Â2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen?¡­ 33920705
Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR¦Á/NF-¦ÊB signaling pathways 34162146
heliyon Dobera glabra (Forssk.) Poir.(Salvadoraceae); phenolic constituents of the aqueous leaves extract and evaluation of its anti-inflammatory, analgesic activities
Open Access Macedonian Journal of Medical Sciences The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation
Annals of the Romanian Society for Cell Biology Comparative Study on the Pro-Inflammatory Activity of Turmeric (Curcuma longa) and flaxseed (Linumusitatissimum)
World J Tradit Chin Med Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement
Veterinary World Comparative evaluation of cytokine and acute-phase protein concentrations in sera of dairy cows with subclinical and clinical ketosis as a different view of the ¡­
biomolecules Treadmill Running Changes Endothelial Lipase Expression: Insights from Gene and Protein Analysis in Various Striated Muscle Tissues and Serum
Int J Biol Macromol Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model 34171261
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus 34395633
PLoS One Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota 34310635
JOURNAL OF ETHNOPHARMACOLOGY Ethanolic extract of Pyrus pashia buch ham ex. D. Don (kainth): A bioaccessible source of polyphenols with anti-inflammatory activity in vitro and in vivo 34517063
Biomed Pharmacother Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats 34426252
CNS Neurosci Ther Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke 34591349
J Biochem Mol Toxicol Thymol ameliorates 5‐fluorouracil‐induced intestinal mucositis: Evidence of down‐regulatory effect on TGF‐β/MAPK pathways through NF‐κB 34665902
Tissue Cell GPNMB plays an active role in the M1/M2 balance 34800878
J Periodontol Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study 34730850
Biofactors Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat 34767670
Inflamm Res Dexmedetomidine exerts cerebral protective effects against cerebral ischemic injury by promoting the polarization of M2 microglia via the Nrf2/HO-1/NLRP3 pathway 34767031
Brain Sci Neuroprotective Effect of Clobenpropit against Lipopolysaccharide-Induced Cognitive Deficits via Attenuating Neuroinflammation and Enhancing Mitochondrial … 34942919
Journal of Immunology Administration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in Rats 34568500
Eur J Pharm Sci Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment 34314841
The Journal of Maternal-Fetal & Neonatal Medicine Expression of Antimicrobial Peptides and Cytokines in Human Omentum Following Abdominal Surgery 34589365
Biomedicines Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes 34829910
Anesthesiology Adverse Mechanical Ventilation and Pneumococcal Pneumonia Induce Immune and Mitochondrial Dysfunctions Mitigated by Mesenchymal Stem Cells in Rabbits Pubmed:34965287
J Inflamm Res Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice Pubmed:35058704
Mol Neurobiol Translocator Protein Ligand PIGA1138 Reduces Disease Symptoms and Severity in Experimental Autoimmune Encephalomyelitis Model of Primary Progressive … Pubmed:35018577
Int J Nanomedicine Protective Effects of Carbon Dots Derived from Armeniacae Semen Amarum Carbonisata Against Acute Lung Injury Induced by Lipopolysaccharides in Rats Pubmed:35023915
Bioengineered Inflammation promotes erythropoietin induced vascular calcification by activating p38 pathway Pubmed:35168476
Toxicology Protection from Benzene-induced Immune Dysfunction in Mice Pubmed:35090963
FASEB J proBDNF/p75NTR promotes rheumatoid arthritis and inflammatory response by activating proinflammatory cytokines Pubmed:35129860
Exp Biol Med (Maywood) Protective antioxidative and anti-inflammatory actions of β-caryophyllene against sulfasalazine-induced nephrotoxicity in rat Pubmed:35068213
Biomed Pharmacother Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis Pubmed:35453007
Drug Des Devel Ther Lung-Protective Effects of Lidocaine Infusion on Patients with Intermediate/High Risk of Postoperative Pulmonary Complications: A Double-Blind Randomized … Pubmed:35422611
Inflammopharmacology Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway Pubmed:35366745
Veterinary Microbiology Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in … Pubmed:35395543
Journal of Nanobiotechnology Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM Pubmed:35351131
J Clin Med Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study Pubmed:35566605
J Nanobiotechnology Cyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in mice Pubmed:35658867
Journal of Biomaterials Applications Photobiomodulation associated with lipid nanoparticles and hyaluronic acid accelerate the healing of excisional wounds Pubmed:35705485
Biomedicines Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model Pubmed:35740356
Viruses Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs Pubmed:35215787
Neuromodulation: Technology at the Neural Interface Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760
Antioxidants Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress Pubmed:35204158
Antioxidants Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory … Pubmed:35204215
Antioxidants Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … Pubmed:35204140
Oxidative Medicine and Cellular Longevity The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis Pubmed:35355860
Frontiers in Molecular Neuroscience Red Nucleus Interleukin-6 Evokes Tactile Allodynia in Male Rats Through Modulating Spinal Pro-inflammatory and Anti-inflammatory Cytokines Pubmed:35465093
Vaccines Age-Dependent Dynamics of Maternally Derived Antibodies (MDAs) and Understanding MDA-Mediated Immune Tolerance in Foot-and-Mouth Disease-Vaccinated … Pubmed:35632433
Journal of Integrative Medicine Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats Pubmed:35643766
researchsquare Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling
Mljekarstvo Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows
Biointerface Research in Applied Chemistry Promising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental …
Protective effects of carbon dots derived from Rhei radix et rhizoma carbonisata on DSS (dextran sulfate sodium)-induced ulcerative colitis
Research Square Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid
Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS
Effectiveness of Carbonic Anhydrase Inhibitor Loaded Nanoparticles in the Treatment of Diabetic Retinopathy
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA056Ga01 Recombinant Interleukin 10 (IL10) Positive Control; Immunogen; SDS-PAGE; WB.
APA056Ga61 Active Interleukin 10 (IL10) Cell culture; Activity Assays.
EPA056Ga61 Eukaryotic Interleukin 10 (IL10) Positive Control; Immunogen; SDS-PAGE; WB.
PAA056Ga01 Polyclonal Antibody to Interleukin 10 (IL10) WB; IHC; ICC; IP.
MAA056Ga21 Monoclonal Antibody to Interleukin 10 (IL10) WB; IHC; ICC; IP.
SEA056Ga ELISA Kit for Interleukin 10 (IL10) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA056Ga Multiplex Assay Kit for Interleukin 10 (IL10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.