≡

H2A Histone Family, Member X (H2AFX)

[Edit]

H2AX

H2A Histone Family, Member X (H2AFX)
CCC Q000
HGNC 4739
MGI 102688
PubMed 18206537
RGD 1566119
UniProt P16104
Wiki H2AFX
H2AX is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin.the gene encodes a 142-amino acid protein having a unique C-terminal sequence, QASQEY, which has been conserved from lower eukaryotes.
Nijmegen breakage syndrome protein (NBS1) and phosphorylated H2AX (gamma-H2AX), which associate with irradiation-induced DNA double-strand breaks (DSBs), are also found at sites of V(D)J (variable, diversity, joining) recombination-induced DSBs. In developing thymocytes, NBS1 and gamma-H2AX form nuclear foci that colocalize with the T-cell receptor-alpha (TCRA) locus in response to recombination-activating gene-1 (RAG1) protein-mediated V(D)J cleavage.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPQ000Hu01 Recombinant H2A Histone Family, Member X (H2AFX) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAQ000Hu01 Polyclonal Antibody to H2A Histone Family, Member X (H2AFX) WB
Assay Kits SEQ000Hu ELISA Kit for H2A Histone Family, Member X (H2AFX) Enzyme-linked immunosorbent assay for Antigen Detection.
SCQ000Hu CLIA Kit for H2A Histone Family, Member X (H2AFX) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant H2A Histone Family, Member X (H2AFX) Recombinant Protein Customized Service Offer
Antibodies PAQ000Mu01 Polyclonal Antibody to H2A Histone Family, Member X (H2AFX) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for H2A Histone Family, Member X (H2AFX) CLIA Kit Customized Service Offer
n/a ELISA Kit for H2A Histone Family, Member X (H2AFX) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant H2A Histone Family, Member X (H2AFX) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to H2A Histone Family, Member X (H2AFX) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to H2A Histone Family, Member X (H2AFX) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for H2A Histone Family, Member X (H2AFX) CLIA Kit Customized Service Offer
n/a ELISA Kit for H2A Histone Family, Member X (H2AFX) ELISA Kit Customized Service Offer
  1. "H2A.X. a histone isoprotein with a conserved C-terminal sequence, is encoded by a novel mRNA with both DNA replication type and polyA 3' processing signals."Nucleic Acids Res. 17:9113-9126(1989) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139."J. Biol. Chem. 273:5858-5868(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Megabase chromatin domains involved in DNA double-strand breaks in vivo."J. Cell Biol. 146:905-916(1999) [PubMed] [Europe PMC] [Abstract]
  5. "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage."Curr. Biol. 10:886-895(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139."J. Biol. Chem. 275:9390-9395(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress."J. Biol. Chem. 276:47759-47762(2001) [PubMed] [Europe PMC] [Abstract]
  8. "NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain."Curr. Biol. 12:1846-1851(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX."J. Biol. Chem. 278:19579-19582(2003) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes."J. Biol. Chem. 278:20303-20312(2003) [PubMed] [Europe PMC] [Abstract]
  11. "MDC1 is a mediator of the mammalian DNA damage checkpoint."Nature 421:961-966(2003) [PubMed] [Europe PMC] [Abstract]
  12. "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner."Nucleic Acids Res. 31:6819-6827(2003) [PubMed] [Europe PMC] [Abstract]
  13. "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation."Cancer Res. 64:2390-2396(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention."EMBO J. 23:2674-2683(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species."J. Biol. Chem. 279:53272-53281(2004) [PubMed] [Europe PMC] [Abstract]
  16. "Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II."J. Biol. Chem. 280:9586-9594(2005) [PubMed] [Europe PMC] [Abstract]
  17. "gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair."Mol. Cell 20:801-809(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Novel function of beta-arrestin2 in the nucleus of mature spermatozoa."J. Cell Sci. 119:3047-3056(2006) [PubMed] [Europe PMC] [Abstract]
  19. "RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins."Cell 131:887-900(2007) [PubMed] [Europe PMC] [Abstract]
  20. "RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly."Cell 131:901-914(2007) [PubMed] [Europe PMC] [Abstract]
  21. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  22. "The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage."Cell 136:420-434(2009) [PubMed] [Europe PMC] [Abstract]
  23. "RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins."Cell 136:435-446(2009) [PubMed] [Europe PMC] [Abstract]
  24. "WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity."Nature 457:57-62(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions."Nature 458:591-596(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  27. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  28. "RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA Damage signaling."Cell 150:1182-1195(2012) [PubMed] [Europe PMC] [Abstract]
  29. "Epstein-Barr virus essential antigen EBNA3C attenuates H2AX expression."J. Virol. 88:3776-3788(2014) [PubMed] [Europe PMC] [Abstract]
  30. "Specific recognition of phosphorylated tail of H2AX by the tandem BRCT domains of MCPH1 revealed by complex structure."J. Struct. Biol. 177:459-468(2012) [PubMed] [Europe PMC] [Abstract]