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PYD And CARD Domain Containing Protein (PYCARD)

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ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1

PYD And CARD Domain Containing Protein (PYCARD)
CCC M075
HGNC 16608
MGI 1931465
PubMed 11498534
RGD 628637
UniProt Q9ULZ3
Wiki PYCARD
PYCARD encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery.The deduced 195-amino acid protein contains an N-terminal pyrin-like domain (PYD) and an 87-residue C-terminal CARD. Fluorescence microscopy demonstrated a ring-like expression in some transfected cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant PYD And CARD Domain Containing Protein (PYCARD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to PYD And CARD Domain Containing Protein (PYCARD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to PYD And CARD Domain Containing Protein (PYCARD) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for PYD And CARD Domain Containing Protein (PYCARD) CLIA Kit Customized Service Offer
n/a ELISA Kit for PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant PYD And CARD Domain Containing Protein (PYCARD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to PYD And CARD Domain Containing Protein (PYCARD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to PYD And CARD Domain Containing Protein (PYCARD) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for PYD And CARD Domain Containing Protein (PYCARD) CLIA Kit Customized Service Offer
n/a ELISA Kit for PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant PYD And CARD Domain Containing Protein (PYCARD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to PYD And CARD Domain Containing Protein (PYCARD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to PYD And CARD Domain Containing Protein (PYCARD) Polyclonal Antibody Customized Service Offer
Assay Kits SEM075Ra ELISA Kit for PYD And CARD Domain Containing Protein (PYCARD) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells."J. Biol. Chem. 274:33835-33838(1999) [PubMed] [Europe PMC] [Abstract]
  2. "TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers."Cancer Res. 60:6236-6242(2000) [PubMed] [Europe PMC] [Abstract]
  3. "A splice variant of ASC regulates IL-1beta release and aggregates differently from intact ASC."Mediators Inflamm. 2009:287387-287387(2009) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1."Cancer Res. 60:6243-6247(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis."Biochem. Biophys. Res. Commun. 284:77-82(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Interaction between pyrin and the apoptotic speck protein (ASC) modulates ASC-induced apoptosis."J. Biol. Chem. 276:39320-39329(2001) [PubMed] [Europe PMC] [Abstract]
  9. "PYPAF1: a PYRIN-containing APAF1-like protein that assembles with ASC and activates NF-kB."J. Biol. Chem. 277:11570-11575(2002) [PubMed] [Europe PMC] [Abstract]
  10. "The PYRIN-CARD protein ASC is an activating adaptor for caspase-1."J. Biol. Chem. 277:21119-21122(2002) [PubMed] [Europe PMC] [Abstract]
  11. "The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways."J. Exp. Med. 196:1605-1615(2002) [PubMed] [Europe PMC] [Abstract]
  12. "The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta."Mol. Cell 10:417-426(2002) [PubMed] [Europe PMC] [Abstract]
  13. "ASC is an activating adaptor for NF-kappa B and caspase-8-dependent apoptosis."Biochem. Biophys. Res. Commun. 303:69-73(2003) [PubMed] [Europe PMC] [Abstract]
  14. "The PAAD/PYRIN-only protein POP1/ASC2 is a modulator of ASC-mediated nuclear-factor-kappa B and pro-caspase-1 regulation."Biochem. J. 373:101-113(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Apoptosis-associated speck-like protein containing a caspase recruitment domain is a regulator of procaspase-1 activation."J. Immunol. 171:6154-6163(2003) [PubMed] [Europe PMC] [Abstract]
  16. "NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder."Immunity 20:319-325(2004) [PubMed] [Europe PMC] [Abstract]
  17. "PYNOD, a novel Apaf-1/CED4-like protein is an inhibitor of ASC and caspase-1."Int. Immunol. 16:777-786(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Cryopyrin-induced interleukin 1beta secretion in monocytic cells: enhanced activity of disease-associated mutants and requirement for ASC."J. Biol. Chem. 279:21924-21928(2004) [PubMed] [Europe PMC] [Abstract]
  19. "PAN1/NALP2/PYPAF2, an inducible inflammatory mediator that regulates NF-kappaB and caspase-1 activation in macrophages."J. Biol. Chem. 279:51897-51907(2004) [PubMed] [Europe PMC] [Abstract]
  20. "ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway."Nat. Cell Biol. 6:121-128(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Role of charged and hydrophobic residues in the oligomerization of the PYRIN domain of ASC."Biochemistry 44:575-583(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Cryopyrin and pyrin activate caspase-1, but not NF-kappaB, via ASC oligomerization."Cell Death Differ. 13:236-249(2006) [PubMed] [Europe PMC] [Abstract]
  23. "ASC directs NF-kappaB activation by regulating receptor interacting protein-2 (RIP2) caspase-1 interactions."J. Immunol. 176:4979-4986(2006) [PubMed] [Europe PMC] [Abstract]
  24. "ASC mediates the induction of multiple cytokines by Porphyromonas gingivalis via caspase-1-dependent and -independent pathways."J. Immunol. 177:4252-4256(2006) [PubMed] [Europe PMC] [Abstract]
  25. "The pyroptosome: a supramolecular assembly of ASC dimers mediating inflammatory cell death via caspase-1 activation."Cell Death Differ. 14:1590-1604(2007) [PubMed] [Europe PMC] [Abstract]
  26. "Reconstituted NALP1 inflammasome reveals two-step mechanism of caspase-1 activation."Mol. Cell 25:713-724(2007) [PubMed] [Europe PMC] [Abstract]
  27. "Mechanism of ASC-mediated apoptosis: bid-dependent apoptosis in type II cells."Oncogene 26:1748-1756(2007) [PubMed] [Europe PMC] [Abstract]
  28. "Cellular pyrin domain-only protein 2 is a candidate regulator of inflammasome activation."Infect. Immun. 75:1484-1492(2007) [PubMed] [Europe PMC] [Abstract]
  29. "Pyrin-only protein 2 modulates NF-kappaB and disrupts ASC:CLR interactions."J. Immunol. 178:3837-3845(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Mapping of POP1-binding site on pyrin domain of ASC."J. Biol. Chem. 283:15390-15398(2008) [PubMed] [Europe PMC] [Abstract]
  31. "Activation of inflammasomes requires intracellular redistribution of the apoptotic speck-like protein containing a caspase recruitment domain."J. Immunol. 182:3173-3182(2009) [PubMed] [Europe PMC] [Abstract]
  32. "Mechanism and repertoire of ASC-mediated gene expression."J. Immunol. 182:7655-7662(2009) [PubMed] [Europe PMC] [Abstract]
  33. "AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA."Nature 458:509-513(2009) [PubMed] [Europe PMC] [Abstract]
  34. "AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC."Nature 458:514-518(2009) [PubMed] [Europe PMC] [Abstract]
  35. "Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes."J. Inflamm. (Lond.) 7:23-23(2010) [PubMed] [Europe PMC] [Abstract]
  36. "Recognition of RNA virus by RIG-I results in activation of CARD9 and inflammasome signaling for interleukin 1 beta production."Nat. Immunol. 11:63-69(2010) [PubMed] [Europe PMC] [Abstract]
  37. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  38. "IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi Sarcoma-associated herpesvirus infection."Cell Host Microbe 9:363-375(2011) [PubMed] [Europe PMC] [Abstract]
  39. "The NLR adaptor ASC/PYCARD regulates DUSP10, mitogen-activated protein kinase (MAPK), and chemokine induction independent of the inflammasome."J. Biol. Chem. 286:19605-19616(2011) [PubMed] [Europe PMC] [Abstract]
  40. "Central and overlapping role of cathepsin B and inflammasome adaptor ASC in antigen presenting function of human dendritic cells."Hum. Immunol. 73:871-878(2012) [PubMed] [Europe PMC] [Abstract]
  41. "A role for mitochondria in NLRP3 inflammasome activation."Nature 469:221-225(2011) [PubMed] [Europe PMC] [Abstract]
  42. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  43. "The death-domain fold of the ASC PYRIN domain, presenting a basis for PYRIN/PYRIN recognition."J. Mol. Biol. 332:1155-1163(2003) [PubMed] [Europe PMC] [Abstract]
  44. "Structure and interdomain dynamics of apoptosis-associated speck-like protein containing a CARD (ASC)."J. Biol. Chem. 284:32932-32941(2009) [PubMed] [Europe PMC] [Abstract]