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Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC)

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HYRC; HYRC1; DNPK1; p350; DNAPK; XRCC7; DNA-PKcs

Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC)
CCC K362
HGNC 9413
MGI 104779
PubMed 22499342
RGD 1308982
UniProt P78527
Wiki PRKDC
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku, which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties. The PRKDC protein showed similarity to phosphatidylinositol 3-kinase family members involved in cell cycle control, DNA repair, and DNA damage responses, and had no detectable activity towards lipids. Other PI kinase proteins involved in DNA repair include FKBP12 and the ataxia-telangiectasia gene (ATM), in which mutations lead to genomic instability and predisposition to cancer and ataxia.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPK362Hu01 Recombinant Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPK362Mu01 Recombinant Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Positive Control; Immunogen; SDS-PAGE; WB.
RPK362Mu02 Recombinant Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAK362Mu01 Polyclonal Antibody to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) WB; IHC; ICC; IP.
PAK362Mu02 Polyclonal Antibody to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) WB; IHC; ICC; IP.
Assay Kits SEK362Mu ELISA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit Customized Service Offer
  1. "DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product."Cell 82:849-856(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Frameshift mutation in PRKDC, the gene for DNA-PKcs, in the DNA repair-defective, human, glioma-derived cell line M059J."Radiat. Res. 156:2-9(2001) [PubMed] [Europe PMC] [Abstract]
  3. "MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13."Cytogenet. Cell Genet. 77:268-270(1997) [PubMed] [Europe PMC] [Abstract]
  4. "Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8."Proc. Natl. Acad. Sci. U.S.A. 92:7515-7519(1995) [PubMed] [Europe PMC] [Abstract]
  5. "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases."J. Immunol. 155:4529-4533(1995) [PubMed] [Europe PMC] [Abstract]
  6. "The human double-stranded DNA-activated protein kinase phosphorylates the 90-kDa heat-shock protein, hsp90 alpha at two NH2-terminal threonine residues."J. Biol. Chem. 264:17275-17280(1989) [PubMed] [Europe PMC] [Abstract]
  7. "A DNA-activated protein kinase from HeLa cell nuclei."Mol. Cell. Biol. 10:6460-6471(1990) [PubMed] [Europe PMC] [Abstract]
  8. "DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein."Eur. J. Biochem. 206:595-603(1992) [PubMed] [Europe PMC] [Abstract]
  9. "The carboxyl-terminal transactivation domain of human serum response factor contains DNA-activated protein kinase phosphorylation sites."J. Biol. Chem. 268:21147-21154(1993) [PubMed] [Europe PMC] [Abstract]
  10. "c-Jun is phosphorylated by the DNA-dependent protein kinase in vitro; definition of the minimal kinase recognition motif."Nucleic Acids Res. 21:1289-1295(1993) [PubMed] [Europe PMC] [Abstract]
  11. "CPP32/Yama/apopain cleaves the catalytic component of DNA-dependent protein kinase in the holoenzyme."FEBS Lett. 393:1-6(1996) [PubMed] [Europe PMC] [Abstract]
  12. "Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs."Gene 175:271-273(1996) [PubMed] [Europe PMC] [Abstract]
  13. "DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2."Cell 91:325-334(1997) [PubMed] [Europe PMC] [Abstract]
  14. "Interaction between DNA-dependent protein kinase and a novel protein, KIP."Mutat. Res. 385:13-20(1997) [PubMed] [Europe PMC] [Abstract]
  15. "Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions."EMBO J. 16:6874-6885(1997) [PubMed] [Europe PMC] [Abstract]
  16. "Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro."J. Biol. Chem. 272:12634-12641(1997) [PubMed] [Europe PMC] [Abstract]
  17. "Inhibition of phosphoinositide 3-kinase related kinases by the radiosensitizing agent wortmannin."Cancer Res. 58:4375-4382(1998) [PubMed] [Europe PMC] [Abstract]
  18. "DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D."Genes Dev. 12:2188-2199(1998) [PubMed] [Europe PMC] [Abstract]
  19. "DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45."J. Biol. Chem. 273:2136-2145(1998) [PubMed] [Europe PMC] [Abstract]
  20. "DNA-dependent protein kinase: DNA binding and activation in the absence of Ku."Proc. Natl. Acad. Sci. U.S.A. 95:525-530(1998) [PubMed] [Europe PMC] [Abstract]
  21. "DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer."Biochemistry 38:1819-1828(1999) [PubMed] [Europe PMC] [Abstract]
  22. "Suppression of the poly(ADP-ribose) polymerase activity by DNA-dependent protein kinase in vitro."Oncogene 18:4616-4625(1999) [PubMed] [Europe PMC] [Abstract]
  23. "Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase."Biochem. J. 368:243-251(2002) [PubMed] [Europe PMC] [Abstract]
  24. "Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination."Cell 108:781-794(2002) [PubMed] [Europe PMC] [Abstract]
  25. "Defining interactions between DNA-PK and ligase IV/XRCC4."DNA Repair 1:225-235(2002) [PubMed] [Europe PMC] [Abstract]
  26. "Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks."Genes Dev. 16:2333-2338(2002) [PubMed] [Europe PMC] [Abstract]
  27. "Werner protein is a target of DNA-dependent protein kinase in vivo and in vitro, and its catalytic activities are regulated by phosphorylation."J. Biol. Chem. 277:18291-18302(2002) [PubMed] [Europe PMC] [Abstract]
  28. "Threonines 2638/2647 in DNA-PK are essential for cellular resistance to ionizing radiation."Cancer Res. 63:1198-1201(2003) [PubMed] [Europe PMC] [Abstract]
  29. "Down-regulation of histone H2B by DNA-dependent protein kinase in response to DNA damage through modulation of octamer transcription factor 1."Cancer Res. 63:7197-7205(2003) [PubMed] [Europe PMC] [Abstract]
  30. "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner."Nucleic Acids Res. 31:6819-6827(2003) [PubMed] [Europe PMC] [Abstract]
  31. "Functional and biochemical dissection of the structure-specific nuclease ARTEMIS."EMBO J. 23:1987-1997(2004) [PubMed] [Europe PMC] [Abstract]
  32. "The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination."J. Exp. Med. 199:315-321(2004) [PubMed] [Europe PMC] [Abstract]
  33. "A biochemically defined system for mammalian nonhomologous DNA end joining."Mol. Cell 16:701-713(2004) [PubMed] [Europe PMC] [Abstract]
  34. "Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response."Mol. Cell. Biol. 24:9207-9220(2004) [PubMed] [Europe PMC] [Abstract]
  35. "DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner."Nucleic Acids Res. 32:1-10(2004) [PubMed] [Europe PMC] [Abstract]
  36. "DNA-PKcs function regulated specifically by protein phosphatase 5."Proc. Natl. Acad. Sci. U.S.A. 101:1247-1252(2004) [PubMed] [Europe PMC] [Abstract]
  37. "DNA-PK is responsible for enhanced phosphorylation of histone H2AX under hypertonic conditions."DNA Repair 4:1172-1181(2005) [PubMed] [Europe PMC] [Abstract]
  38. "Artemis deficiency confers a DNA double-strand break repair defect and Artemis phosphorylation status is altered by DNA damage and cell cycle progression."DNA Repair 4:556-570(2005) [PubMed] [Europe PMC] [Abstract]
  39. "The Artemis:DNA-PKcs endonuclease cleaves DNA loops, flaps, and gaps."DNA Repair 4:845-851(2005) [PubMed] [Europe PMC] [Abstract]
  40. "Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage."Nature 434:605-611(2005) [PubMed] [Europe PMC] [Abstract]
  41. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  42. "XRCC1 is phosphorylated by DNA-dependent protein kinase in response to DNA damage."Nucleic Acids Res. 34:32-41(2006) [PubMed] [Europe PMC] [Abstract]
  43. "The life and death of DNA-PK."Oncogene 24:949-961(2005) [PubMed] [Europe PMC] [Abstract]
  44. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  45. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  46. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  47. "A DNA-PKcs mutation in a radiosensitive T-B- SCID patient inhibits Artemis activation and nonhomologous end-joining."J. Clin. Invest. 119:91-98(2009) [PubMed] [Europe PMC] [Abstract]
  48. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  49. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  50. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  51. "A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability."Genes Dev. 24:1939-1950(2010) [PubMed] [Europe PMC] [Abstract]
  52. "Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes."Genes Dev. 24:2019-2030(2010) [PubMed] [Europe PMC] [Abstract]
  53. "Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly."J. Biol. Chem. 285:20109-20116(2010) [PubMed] [Europe PMC] [Abstract]
  54. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  55. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  56. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  57. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
  58. "Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response."Mol. Cell. Biol. 33:406-417(2013) [PubMed] [Europe PMC] [Abstract]
  59. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  60. "RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage."Proc. Natl. Acad. Sci. U.S.A. 111:E2646-E2655(2014) [PubMed] [Europe PMC] [Abstract]
  61. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  62. "PRKDC mutations in a SCID patient with profound neurological abnormalities."J. Clin. Invest. 123:2969-2980(2013) [PubMed] [Europe PMC] [Abstract]