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Anillin (ANLN)

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Scra; Scraps; Actin-Binding Protein Anillin

Anillin (ANLN)
CCC J640
HGNC 14082
MGI 1920174
PubMed 7559773
RGD 1566097
UniProt Q9NQW6
Wiki ANLN
Anillin migrates at 190 kD on SDS-PAGE. Anillin is expressed throughout Drosophila development and in tissue culture cells. In contractile rings, metaphase furrows, and nascent ring canals, anillin remains bound to the invaginated cortex suggesting a stabilizing role. Anillin is not expressed in cells that have left the cell cycle. Anillin isolated from embryo extracts binds directly to actin filaments. The domain responsible for this binding has been mapped to a region of 244 amino acids by expression of protein fragments in bacteria. This domain, which is monomeric in solution, also bundles actin filaments. Anillin plays a role in organizing and/or stabilizing the cleavage furrow and other cell cycle regulated, contractile domains of the actin cytoskeleton.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ640Hu01 Recombinant Anillin (ANLN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ640Hu01 Polyclonal Antibody to Anillin (ANLN) WB; IHC; ICC; IP.
MAJ640Hu21 Monoclonal Antibody to Anillin (ANLN) WB; IHC; ICC; IP.
Assay Kits SEJ640Hu ELISA Kit for Anillin (ANLN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Anillin (ANLN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Anillin (ANLN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Anillin (ANLN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Anillin (ANLN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Anillin (ANLN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Anillin (ANLN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Anillin (ANLN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Anillin (ANLN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Anillin (ANLN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Anillin (ANLN) ELISA Kit Customized Service Offer
  1. "Functional analysis of a human homolog of the Drosophila actin binding protein anillin suggests a role in cytokinesis."J. Cell Biol. 150:539-552(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Self- and actin-templated assembly of mammalian septins."Dev. Cell 3:791-802(2002) [PubMed] [Europe PMC] [Abstract]
  6. "Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor."Science 299:1743-1747(2003) [PubMed] [Europe PMC] [Abstract]
  7. "ANLN plays a critical role in human lung carcinogenesis through the activation of RHOA and by involvement in the phosphoinositide 3-kinase/AKT pathway."Cancer Res. 65:11314-11325(2005) [PubMed] [Europe PMC] [Abstract]
  8. "The septin-binding protein anillin is overexpressed in diverse human tumors."Clin. Cancer Res. 11:6780-6786(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Anillin is a substrate of anaphase-promoting complex/cyclosome (APC/C) that controls spatial contractility of myosin during late cytokinesis."J. Biol. Chem. 280:33516-33524(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Anillin binds nonmuscle myosin II and regulates the contractile ring."Mol. Biol. Cell 16:193-201(2005) [PubMed] [Europe PMC] [Abstract]
  11. "Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not."Mol. Biol. Cell 16:1043-1055(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Clues to CD2-associated protein involvement in cytokinesis."Mol. Biol. Cell 16:2891-2902(2005) [PubMed] [Europe PMC] [Abstract]
  13. "MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis."Proc. Natl. Acad. Sci. U.S.A. 102:13158-13163(2005) [PubMed] [Europe PMC] [Abstract]
  14. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  17. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  23. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  24. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  25. "Mutations in the gene that encodes the F-actin binding protein anillin cause FSGS." J. Am. Soc. Nephrol. 25:1991-2002(2014) [PubMed] [Europe PMC] [Abstract]