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Dihydroorotate Dehydrogenase (DHODH)

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DHOdehase; Dihydroorotate oxidase; Dihydroorotate dehydrogenase (quinone), mitochondrial

Dihydroorotate Dehydrogenase (DHODH)
CCC J271
HGNC 2867
MGI 1928378
PubMed 21430780
RGD 68352
UniProt Q02127
Wiki DHODH
Dihydroorotate dehydrogenase (EC 1.3.3.1) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis. Minet et al. (1992) isolated a truncated human cDNA encoding this enzyme from a HeLa cell cDNA library by functional complementation of a corresponding deletion mutant from the Saccharomyces cerevisiae. The gene in yeast is symbolized ura1. DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane. In yeast, however, it has a cytosolic location.
Missense mutations of the DHODH gene cause Miller syndrome, an autosomal recessive disorder also known as postaxial acrofacial dysostosis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ271Hu01 Recombinant Dihydroorotate Dehydrogenase (DHODH) Positive Control; Immunogen; SDS-PAGE; WB.
APJ271Hu01 Active Dihydroorotate Dehydrogenase (DHODH) Cell culture; Activity Assays.
Antibodies PAJ271Hu01 Polyclonal Antibody to Dihydroorotate Dehydrogenase (DHODH) WB; IHC; ICC; IP.
MAJ271Hu21 Monoclonal Antibody to Dihydroorotate Dehydrogenase (DHODH) WB; IHC; ICC; IP.
Assay Kits SEJ271Hu ELISA Kit for Dihydroorotate Dehydrogenase (DHODH) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ271Mu01 Recombinant Dihydroorotate Dehydrogenase (DHODH) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ271Mu02 Recombinant Dihydroorotate Dehydrogenase (DHODH) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ271Mu01 Polyclonal Antibody to Dihydroorotate Dehydrogenase (DHODH) WB; IHC; ICC; IP.
PAJ271Mu02 Polyclonal Antibody to Dihydroorotate Dehydrogenase (DHODH) WB; IHC; ICC; IP.
Assay Kits SEJ271Mu ELISA Kit for Dihydroorotate Dehydrogenase (DHODH) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Dihydroorotate Dehydrogenase (DHODH) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Dihydroorotate Dehydrogenase (DHODH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Dihydroorotate Dehydrogenase (DHODH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Dihydroorotate Dehydrogenase (DHODH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Dihydroorotate Dehydrogenase (DHODH) ELISA Kit Customized Service Offer
  1. "Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant."Gene 121:393-396(1992) [PubMed] [Europe PMC] [Abstract]
  2. "Recombinant human dihydroorotate dehydrogenase: expression, purification, and characterization of a catalytically functional truncated enzyme."Arch. Biochem. Biophys. 323:79-86(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme."Eur. J. Biochem. 240:292-301(1996) [PubMed] [Europe PMC] [Abstract]
  6. "Requirements for the mitochondrial import and localization of dihydroorotate dehydrogenase."Eur. J. Biochem. 267:2079-2087(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  9. "Structures of human dihydroorotate dehydrogenase in complex with antiproliferative agents."Structure 8:25-33(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Dual binding mode of a novel series of DHODH inhibitors."J. Med. Chem. 49:1239-1247(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Exome sequencing identifies the cause of a Mendelian disorder."Nat. Genet. 42:30-35(2010) [PubMed] [Europe PMC] [Abstract]