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Polyribonucleotide Nucleotidyltransferase 1 (PNPT1)

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PNPase; OLD35; Polynucleotide Phosphorylase; 3'-5' RNA Exonuclease; 3'-5' RNA exonuclease OLD35; Polynucleotide phosphorylase-like protein

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1)
CCC G859
HGNC 23166
MGI 1918951
PubMed 23084290
RGD 1307987
UniProt Q8TCS8
PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation.
The variants differ only in the length of their 3-prime UTRs, and both encode a deduced 783-amino acid protein with a calculated molecular mass of 86 kD. PNPT1 contains 2 RNase PH domains, a KH domain, and a C-terminal S1 domain. Northern blot analysis detected 4.0- and 2.6-kb transcripts in terminally differentiated human melanoma cells.Expression of PNPT1 was induced by interferon-beta in a human metastatic melanoma cell line, and expression of PNPT1 suppressed melanoma cell colony formation.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Polyclonal Antibody Customized Service Offer
Assay Kits SEG859Hu ELISA Kit for Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polyribonucleotide Nucleotidyltransferase 1 (PNPT1) ELISA Kit Customized Service Offer
  1. "Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring."J. Mol. Biol. 323:653-663(2002) [PubMed] [Europe PMC] [Abstract]
  2. "Identification and cloning of human polynucleotide phosphorylase, hPNPase (old-35), in the context of terminal differentiation and cellular senescence."Proc. Natl. Acad. Sci. U.S.A. 99:16636-16641(2002) [PubMed] [Europe PMC] [Abstract]
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  6. "Down-regulation of Myc as a potential target for growth arrest induced by human polynucleotide phosphorylase (hPNPaseold-35) in human melanoma cells."J. Biol. Chem. 278:24542-24551(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria."J. Mol. Biol. 329:853-857(2003) [PubMed] [Europe PMC] [Abstract]
  8. "Defining the domains of human polynucleotide phosphorylase (hPNPaseOLD-35) mediating cellular senescence."Mol. Cell. Biol. 25:7333-7343(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Defining the mechanism by which IFN-beta dowregulates c-myc expression in human melanoma cells: pivotal role for human polynucleotide phosphorylase (hPNPaseold-35)."Cell Death Differ. 13:1541-1553(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Mammalian polynucleotide phosphorylase is an intermembrane space RNase that maintains mitochondrial homeostasis."Mol. Cell. Biol. 26:8475-8487(2006) [PubMed] [Europe PMC] [Abstract]
  11. "The TCL1 oncoprotein binds the RNase PH domains of the PNPase exoribonuclease without affecting its RNA degrading activity."Cancer Lett. 248:198-210(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Human polynucleotide phosphorylase reduces oxidative RNA damage and protects HeLa cell against oxidative stress."Biochem. Biophys. Res. Commun. 372:288-292(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Analysis of the human polynucleotide phosphorylase (PNPase) reveals differences in RNA binding and response to phosphate compared to its bacterial and chloroplast counterparts."RNA 14:297-309(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Stable PNPase RNAi silencing: its effect on the processing and adenylation of human mitochondrial RNA."RNA 14:310-323(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Human mitochondrial SUV3 and polynucleotide phosphorylase form a 330-kDa heteropentamer to cooperatively degrade double-stranded RNA with a 3'-to-5' directionality."J. Biol. Chem. 284:20812-20821(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  17. "PNPASE regulates RNA import into mitochondria."Cell 142:456-467(2010) [PubMed] [Europe PMC] [Abstract]
  18. "Human polynucleotide phosphorylase selectively and preferentially degrades microRNA-221 in human melanoma cells."Proc. Natl. Acad. Sci. U.S.A. 107:11948-11953(2010) [PubMed] [Europe PMC] [Abstract]
  19. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Mutation in PNPT1, which encodes a polyribonucleotide nucleotidyltransferase, impairs RNA import into mitochondria and causes respiratory-chain deficiency."Am. J. Hum. Genet. 91:912-918(2012) [PubMed] [Europe PMC] [Abstract]
  22. "A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss." Am. J. Hum. Genet. 91:919-927(2012) [PubMed] [Europe PMC] [Abstract]