Discs, Large Homolog 1 (DLG1)

[Edit]

DLGH1; SAP97; Hdlg; Presynaptic Protein SAP97; Synapse-Associated Protein 97

Discs, Large Homolog 1 (DLG1)
SAP97 is a mammalian MAGUK-family member protein that is similar to the Drosophila protein Dlg1 (the protein is alternatively referred to as hDlg1, and the human gene is DLG1). SAP97 is expressed throughout the body in epithelial cells. In the brain it is involved in the trafficking of ionotropic receptors from the endoplasmic Reticulum to the plasma membrane, and may be involved in the trafficking AMPAR during synaptic plasticity.
SAP97's protein structure consists of an alternatively-spliced n-terminal domain, three PDZ domains, an SH3 domain, hook domain, I3 domain, and finally an inactive guanylate kinase (GK) domain. Each of these domains has specific interacting partners that help define SAP97's unique function.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG169Hu01 Recombinant Discs, Large Homolog 1 (DLG1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Discs, Large Homolog 1 (DLG1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Discs, Large Homolog 1 (DLG1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Discs, Large Homolog 1 (DLG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Discs, Large Homolog 1 (DLG1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Discs, Large Homolog 1 (DLG1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Discs, Large Homolog 1 (DLG1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Discs, Large Homolog 1 (DLG1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Discs, Large Homolog 1 (DLG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Discs, Large Homolog 1 (DLG1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG169Ra01 Recombinant Discs, Large Homolog 1 (DLG1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Discs, Large Homolog 1 (DLG1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Discs, Large Homolog 1 (DLG1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Discs, Large Homolog 1 (DLG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Discs, Large Homolog 1 (DLG1) ELISA Kit Customized Service Offer
  1. "Cloning and characterization of hdlg: the human homologue of the Drosophila discs large tumor suppressor binds to protein 4.1."Proc. Natl. Acad. Sci. U.S.A. 91:9818-9822(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence, annotation and analysis of human chromosome 3." Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Two independent domains of hDlg are sufficient for subcellular targeting: the PDZ1-2 conformational unit and an alternatively spliced domain."J. Cell Biol. 135:1125-1137(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Binding of APC to the human homolog of the Drosophila discs large tumor suppressor protein."Science 272:1020-1023(1996) [PubMed] [Europe PMC] [Abstract]
  9. "Binding of human virus oncoproteins to hDlg/SAP97, a mammalian homolog of the Drosophila discs large tumor suppressor protein."Proc. Natl. Acad. Sci. U.S.A. 94:6670-6675(1997) [PubMed] [Europe PMC] [Abstract]
  10. "Tax oncoprotein of HTLV-1 binds to the human homologue of Drosophila discs large tumor suppressor protein, hDLG, and perturbs its function in cell growth control."Oncogene 18:5967-5972(1999) [PubMed] [Europe PMC] [Abstract]
  11. "GAKIN, a novel kinesin-like protein associates with the human homologue of the Drosophila discs large tumor suppressor in T lymphocytes."J. Biol. Chem. 275:28774-28784(2000) [PubMed] [Europe PMC] [Abstract]
  12. "The APC-hDLG complex negatively regulates cell cycle progression from the G0/G1 to S phase."Oncogene 19:365-372(2000) [PubMed] [Europe PMC] [Abstract]
  13. "Characterization of PDZ-binding kinase, a mitotic kinase."Proc. Natl. Acad. Sci. U.S.A. 97:5167-5172(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells."J. Biol. Chem. 276:48350-48355(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Expression, regulation and role of the MAGUK protein SAP-97 in human atrial myocardium."Cardiovasc. Res. 56:433-442(2002) [PubMed] [Europe PMC] [Abstract]
  16. "The distribution and function of alternatively spliced insertions in hDlg."J. Biol. Chem. 277:6406-6412(2002) [PubMed] [Europe PMC] [Abstract]
  17. "Protein 4.1-mediated membrane targeting of human discs large in epithelial cells."J. Biol. Chem. 278:34445-34450(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Human homolog of disc-large is required for adherens junction assembly and differentiation of human intestinal epithelial cells."J. Biol. Chem. 279:10157-10166(2004) [PubMed] [Europe PMC] [Abstract]
  19. "Discs large (Dlg1) complexes in lymphocyte activation."J. Cell Biol. 166:173-178(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein."Oncogene 23:3889-3897(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Dlg, Scribble and Lgl in cell polarity, cell proliferation and cancer."Bioessays 25:542-553(2003) [PubMed] [Europe PMC] [Abstract]
  22. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  23. "SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses."Neuron 50:233-245(2006) [PubMed] [Europe PMC] [Abstract]
  24. "The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions."J. Biol. Chem. 282:9392-9400(2007) [PubMed] [Europe PMC] [Abstract]
  25. "The MAGUK protein MPP7 binds to the polarity protein hDlg1 and facilitates epithelial tight junction formation."Mol. Biol. Cell 18:1744-1755(2007) [PubMed] [Europe PMC] [Abstract]
  26. "Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis."J. Neurosci. 28:14546-14556(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  28. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  29. "Kv4 potassium channels form a tripartite complex with the anchoring protein SAP97 and CaMKII in cardiac myocytes."Circ. Res. 104:758-769(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  31. "p38gamma regulates interaction of nuclear PSF and RNA with the tumour-suppressor hDlg in response to osmotic shock."J. Cell Sci. 123:2596-2604(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  34. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  35. "Crystal structure of a PDZ domain."Nature 382:649-652(1996) [PubMed] [Europe PMC] [Abstract]
  36. "Structural basis for tandem L27 domain-mediated polymerization."FASEB J. 24:4806-4815(2010) [PubMed] [Europe PMC] [Abstract]