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Thymidylate kinase (EC 2.7.4.9; ATP:dTMP phosphotransferase) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis. Knowledge of the essential function of dTMP kinase in eukaryotes comes from the study of a cell cycle mutant, cdc8, in Saccharomyces cerevisiae. The product of the cdc8 gene is involved in the elongation step of S phase in cell cycle progression. Huang et al. (1994) cloned the human thymidylate kinase gene by complementation with the cdc8 mutation. The cDNA sequence revealed an open reading frame that encodes a protein with a molecular weight of 23,806. Structural and functional analyses suggested that the cDNA codes for authentic human dTMP kinase. The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages.
Organism species: Homo sapiens (Human)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPD780Hu01 | Recombinant Deoxythymidylate Kinase (DTYMK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAD780Hu01 | Polyclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. |
| Assay Kits | n/a | CLIA Kit for Deoxythymidylate Kinase (DTYMK) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Deoxythymidylate Kinase (DTYMK) | ELISA Kit Customized Service Offer |
Organism species: Mus musculus (Mouse)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPD780Mu01 | Recombinant Deoxythymidylate Kinase (DTYMK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAD780Mu01 | Polyclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. |
| MAD780Mu21 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. | |
| MAD780Mu22 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB | |
| MAD780Mu23 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. | |
| MAD780Mu24 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. | |
| MAD780Mu25 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. | |
| MAD780Mu27 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. | |
| MAD780Mu29 | Monoclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. | |
| Assay Kits | n/a | CLIA Kit for Deoxythymidylate Kinase (DTYMK) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Deoxythymidylate Kinase (DTYMK) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPD780Ra01 | Recombinant Deoxythymidylate Kinase (DTYMK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAD780Ra01 | Polyclonal Antibody to Deoxythymidylate Kinase (DTYMK) | WB; IHC; ICC; IP. |
| Assay Kits | n/a | CLIA Kit for Deoxythymidylate Kinase (DTYMK) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Deoxythymidylate Kinase (DTYMK) | ELISA Kit Customized Service Offer |
- "Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast."Nucleic Acids Res. 19:823-827(1991) [PubMed] [Europe PMC] [Abstract]
- "Human dTMP kinase: gene expression and enzymatic activity coinciding with cell cycle progression and cell growth."DNA Cell Biol. 13:461-471(1994) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
- "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
- "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
- "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
- "Structures of human thymidylate kinase in complex with prodrugs: implications for the structure-based design of novel compounds."Biochemistry 42:2568-2577(2003) [PubMed] [Europe PMC] [Abstract]