Sequestosome 1 (SQSTM1)

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OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62

Sequestosome 1 (SQSTM1)
The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine. p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. A 62-kD protein (Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa) was identified by Park et al. (1995) as a ligand of the p56-lck SH2 domain. Gong et al. (1999) screened a yeast 2-hybrid library made from rat hippocampal mRNA against full-length Kv-beta-2 . Two previously unknown interacting proteins were identified, one of which was identical to Zip (PKC-zeta)-interacting protein), the rat homolog of p62. Zip1 and Zip2, which arise from alternative splicing, are identical except for 27 residues missing from Zip2.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD198Hu01 Recombinant Sequestosome 1 (SQSTM1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD198Hu01 Polyclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
MAD198Hu21 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
MAD198Hu22 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
MAD198Hu23 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
MAD198Hu24 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
MAD198Hu27 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
MAD198Hu25 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
Assay Kits SED198Hu ELISA Kit for Sequestosome 1 (SQSTM1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD198Mu01 Recombinant Sequestosome 1 (SQSTM1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Sequestosome 1 (SQSTM1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Sequestosome 1 (SQSTM1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Sequestosome 1 (SQSTM1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Sequestosome 1 (SQSTM1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD198Ra01 Recombinant Sequestosome 1 (SQSTM1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD198Ra01 Polyclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
Assay Kits SED198Ra ELISA Kit for Sequestosome 1 (SQSTM1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes."J. Virol. 70:1143-1153(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain."Proc. Natl. Acad. Sci. U.S.A. 93:5991-5995(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Genomic structure and promoter analysis of the p62 gene encoding a non-proteasomal multiubiquitin chain binding protein."FEBS Lett. 435:138-142(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent."Am. J. Pathol. 154:1701-1710(1999) [PubMed] [Europe PMC] [Abstract]
  8. "Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56lck and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal region."Proc. Natl. Acad. Sci. U.S.A. 92:12338-12342(1995) [PubMed] [Europe PMC] [Abstract]
  9. "p62, a phosphotyrosine-independent ligand of the SH2 domain of p56lck, belongs to a new class of ubiquitin-binding proteins."J. Biol. Chem. 271:20235-20237(1996) [PubMed] [Europe PMC] [Abstract]
  10. "A p56(lck) ligand serves as a coactivator of an orphan nuclear hormone receptor."J. Biol. Chem. 271:27197-27200(1996) [PubMed] [Europe PMC] [Abstract]
  11. "Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62."Mol. Cell. Biol. 18:3069-3080(1998) [PubMed] [Europe PMC] [Abstract]
  12. "The interaction of p62 with RIP links the atypical PKCs to NF-kappaB activation."EMBO J. 18:3044-3053(1999) [PubMed] [Europe PMC] [Abstract]
  13. "p62 functions as a p38 MAP kinase regulator."Biochem. Biophys. Res. Commun. 269:521-525(2000) [PubMed] [Europe PMC] [Abstract]
  14. "The atypical PKC-interacting protein p62 channels NF-kappaB activation by the IL-1-TRAF6 pathway."EMBO J. 19:1576-1586(2000) [PubMed] [Europe PMC] [Abstract]
  15. "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor."J. Biol. Chem. 276:7709-7712(2001) [PubMed] [Europe PMC] [Abstract]
  16. "p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases."Am. J. Pathol. 160:255-263(2002) [PubMed] [Europe PMC] [Abstract]
  17. "p62 forms a ternary complex with PKCzeta and PAR-4 and antagonizes PAR-4-induced PKCzeta inhibition."FEBS Lett. 510:57-61(2002) [PubMed] [Europe PMC] [Abstract]
  18. "Mallory body -- a disease-associated type of sequestosome."Hepatology 35:1053-1062(2002) [PubMed] [Europe PMC] [Abstract]
  19. "Association of the atypical protein kinase C-interacting protein p62/ZIP with nerve growth factor receptor TrkA regulates receptor trafficking and Erk5 signaling."J. Biol. Chem. 278:4730-4739(2003) [PubMed] [Europe PMC] [Abstract]
  20. "Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins."J. Biol. Chem. 278:34568-34581(2003) [PubMed] [Europe PMC] [Abstract]
  21. "PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62."Mol. Cell 12:39-50(2003) [PubMed] [Europe PMC] [Abstract]
  22. "p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells."Oncogene 22:2322-2333(2003) [PubMed] [Europe PMC] [Abstract]
  23. "Transcriptional activation of p62/A170/ZIP during the formation of the aggregates: possible mechanisms and the role in Lewy body formation in Parkinson's disease."Brain Res. 1012:42-51(2004) [PubMed] [Europe PMC] [Abstract]
  24. "Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation."Mol. Cell. Biol. 24:8055-8068(2004) [PubMed] [Europe PMC] [Abstract]
  25. "The p62 scaffold regulates nerve growth factor-induced NF-kappaB activation by influencing TRAF6 polyubiquitination."J. Biol. Chem. 280:35625-35629(2005) [PubMed] [Europe PMC] [Abstract]
  26. "p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death."J. Cell Biol. 171:603-614(2005) [PubMed] [Europe PMC] [Abstract]
  27. "Sequestosome 1/p62 shuttles polyubiquitinated tau for proteasomal degradation."J. Neurochem. 94:192-203(2005) [PubMed] [Europe PMC] [Abstract]
  28. "The LIM protein Ajuba influences interleukin-1-induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/p62/TRAF6 signaling complex."Mol. Cell. Biol. 25:4010-4022(2005) [PubMed] [Europe PMC] [Abstract]
  29. "Inhibition of sequestosome 1/p62 up-regulation prevents aggregation of ubiquitinated proteins induced by prostaglandin J2 without reducing its neurotoxicity."Mol. Cell. Neurosci. 29:222-231(2005) [PubMed] [Europe PMC] [Abstract]
  30. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  31. "The kinase domain of titin controls muscle gene expression and protein turnover."Science 308:1599-1603(2005) [PubMed] [Europe PMC] [Abstract]
  32. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  33. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  34. "p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy."J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract]
  35. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  36. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  37. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  38. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  39. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  40. "p62/SQSTM1 and ALFY interact to facilitate the formation of p62 bodies/ALIS and their degradation by autophagy."Autophagy 6:330-344(2010) [PubMed] [Europe PMC] [Abstract]
  41. "Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance."J. Cell Biol. 191:1159-1172(2010) [PubMed] [Europe PMC] [Abstract]
  42. "p62/sequestosome-1 associates with and sustains the expression of retroviral restriction factor TRIM5alpha."J. Virol. 84:5997-6006(2010) [PubMed] [Europe PMC] [Abstract]
  43. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  44. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  45. "Independent interactions of ubiquitin-binding domains in a ubiquitin-mediated ternary complex."Biochemistry 50:9076-9087(2011) [PubMed] [Europe PMC] [Abstract]
  46. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  47. "TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells."Biochim. Biophys. Acta 1823:316-326(2012) [PubMed] [Europe PMC] [Abstract]
  48. "TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death."Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract]
  49. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  50. "TRAF6 mediates ubiquitination of KIF23/MKLP1 and is required for midbody ring degradation by selective autophagy."Autophagy 9:1955-1964(2013) [PubMed] [Europe PMC] [Abstract]
  51. "The LIR motif - crucial for selective autophagy."J. Cell Sci. 126:3237-3247(2013) [PubMed] [Europe PMC] [Abstract]
  52. "Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites."Nature 502:254-257(2013) [PubMed] [Europe PMC] [Abstract]
  53. "Structural determinants in GABARAP required for the selective binding and recruitment of ALFY to LC3B-positive structures."EMBO Rep. 15:557-565(2014) [PubMed] [Europe PMC] [Abstract]
  54. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  55. "Structure of the ubiquitin-associated domain of p62 (SQSTM1) and implications for mutations that cause Paget's disease of bone."J. Biol. Chem. 278:37409-37412(2003) [PubMed] [Europe PMC] [Abstract]
  56. "Ubiquitin recognition by the ubiquitin-associated domain of p62 involves a novel conformational switch."J. Biol. Chem. 283:5427-5440(2008) [PubMed] [Europe PMC] [Abstract]
  57. "Conformation and dynamics of the three-helix bundle UBA domain of p62 from experiment and simulation."Proteins 71:227-240(2008) [PubMed] [Europe PMC] [Abstract]
  58. "Dimerisation of the UBA domain of p62 inhibits ubiquitin binding and regulates NF-kappaB signalling."J. Mol. Biol. 396:178-194(2010) [PubMed] [Europe PMC] [Abstract]
  59. "Recurrent mutation of the gene encoding sequestosome 1 (SQSTM1/p62) in Paget disease of bone."Am. J. Hum. Genet. 70:1582-1588(2002) [PubMed] [Europe PMC] [Abstract]
  60. "Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease."Hum. Mol. Genet. 11:2735-2739(2002) [PubMed] [Europe PMC] [Abstract]
  61. "Three novel mutations in SQSTM1 identified in familial Paget's disease of bone."J. Bone Miner. Res. 18:1748-1753(2003) [PubMed] [Europe PMC] [Abstract]
  62. "Familial Paget's disease in The Netherlands: occurrence, identification of new mutations in the sequestosome 1 gene, and their clinical associations."Arthritis Rheum. 50:1650-1654(2004) [PubMed] [Europe PMC] [Abstract]
  63. "Identification of SQSTM1 mutations in familial Paget's disease in Australian pedigrees."Bone 35:277-282(2004) [PubMed] [Europe PMC] [Abstract]
  64. "Two novel mutations at exon 8 of the Sequestosome 1 (SQSTM1) gene in an Italian series of patients affected by Paget's disease of bone (PDB)."J. Bone Miner. Res. 19:1013-1017(2004) [PubMed] [Europe PMC] [Abstract]
  65. "Novel UBA domain mutations of SQSTM1 in Paget's disease of bone: genotype phenotype correlation, functional analysis, and structural consequences."J. Bone Miner. Res. 19:1122-1127(2004) [PubMed] [Europe PMC] [Abstract]
  66. "Detection and validation of non-synonymous coding SNPs from orthogonal analysis of shotgun proteomics data."J. Proteome Res. 6:2331-2340(2007) [PubMed] [Europe PMC] [Abstract]