≡

Serine/threonine-protein kinase mTOR (mTOR)

[Edit]

FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target

Serine/threonine-protein kinase mTOR (mTOR)
CCC B806
HGNC 3942
MGI 1928394
PubMed 11146621
RGD 68371
UniProt P42345
Wiki FRAP

The mammalian target of rapamycin (mTOR) also known as FK506 binding protein 12-rapamycin associated protein 1 (FRAP1) is a protein which in humans is encoded by the FRAP1 gene.mTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription.

Current research indicates that mTOR integrates the input from multiple upstream pathways, including insulin, growth factors (such as IGF-1 and IGF-2), and mitogens.mTOR also functions as a sensor of cellular nutrient and energy levels and redox status.The disregulation of the mTOR pathway is implicated as a contributing factor to various human disease processes, especially various types of cancer.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB806Hu01 Recombinant Serine/threonine-protein kinase mTOR (mTOR) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB806Hu01 Polyclonal Antibody to Serine/threonine-protein kinase mTOR (mTOR) WB; IHC; ICC; IP.
MAB806Hu21 Monoclonal Antibody to Serine/threonine-protein kinase mTOR (mTOR) WB; IHC
Assay Kits SEB806Hu ELISA Kit for Serine/threonine-protein kinase mTOR (mTOR) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB806Mu01 Recombinant Serine/threonine-protein kinase mTOR (mTOR) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Serine/threonine-protein kinase mTOR (mTOR) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Serine/threonine-protein kinase mTOR (mTOR) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Serine/threonine-protein kinase mTOR (mTOR) CLIA Kit Customized Service Offer
n/a ELISA Kit for Serine/threonine-protein kinase mTOR (mTOR) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Serine/threonine-protein kinase mTOR (mTOR) Recombinant Protein Customized Service Offer
Antibodies PAB806Ra01 Polyclonal Antibody to Serine/threonine-protein kinase mTOR (mTOR) WB; IHC; ICC; IP.
Assay Kits SEB806Ra ELISA Kit for Serine/threonine-protein kinase mTOR (mTOR) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "A mammalian protein targeted by G1-arresting rapamycin-receptor complex."Nature 369:756-758(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and expression analysis of five novel genes in chromosome 1p36."Genomics 50:187-198(1998) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The human gene for mannan-binding lectin-associated serine protease-2 (MASP-2), the effector component of the lectin route of complement activation, is part of a tightly linked gene cluster on chromosome 1p36.2-3."Genes Immun. 2:119-127(2001) [PubMed] [Europe PMC] [Abstract]
  6. "RAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex."Proc. Natl. Acad. Sci. U.S.A. 91:12574-12578(1994) [PubMed] [Europe PMC] [Abstract]
  7. "Expression, enzyme activity, and subcellular localization of mammalian target of rapamycin in insulin-responsive cells."Biochem. Biophys. Res. Commun. 241:704-709(1997) [PubMed] [Europe PMC] [Abstract]
  8. "Characterization of ubiquilin 1, an mTOR-interacting protein."Biochim. Biophys. Acta 1542:41-56(2002) [PubMed] [Europe PMC] [Abstract]
  9. "mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the growth machinery."Cell 110:163-175(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action."Cell 110:177-189(2002) [PubMed] [Europe PMC] [Abstract]
  11. "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase."EMBO Rep. 3:988-994(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Regulation of ribosomal S6 kinase 2 by mammalian target of rapamycin."J. Biol. Chem. 277:31423-31429(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
  14. "FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction."Proc. Natl. Acad. Sci. U.S.A. 99:4319-4324(2002) [PubMed] [Europe PMC] [Abstract]
  15. "TSC2 mediates cellular energy response to control cell growth and survival."Cell 115:577-590(2003) [PubMed] [Europe PMC] [Abstract]
  16. "GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR."Mol. Cell 11:895-904(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton."Curr. Biol. 14:1296-1302(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex."Genes Dev. 18:2893-2904(2004) [PubMed] [Europe PMC] [Abstract]
  19. "FKBP12-rapamycin-associated protein or mammalian target of rapamycin (FRAP/mTOR) localization in the endoplasmic reticulum and the Golgi apparatus."J. Biol. Chem. 279:772-778(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive."Nat. Cell Biol. 6:1122-1128(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase."J. Biol. Chem. 280:26089-26093(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex."Science 307:1098-1101(2005) [PubMed] [Europe PMC] [Abstract]
  23. "PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor beta expression and signaling."J. Biol. Chem. 282:25604-25612(2007) [PubMed] [Europe PMC] [Abstract]
  24. "PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase."Mol. Cell 25:903-915(2007) [PubMed] [Europe PMC] [Abstract]
  25. "mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1)."Biochem. J. 416:375-385(2008) [PubMed] [Europe PMC] [Abstract]
  26. "SREBP activity is regulated by mTORC1 and contributes to Akt-dependent cell growth."Cell Metab. 8:224-236(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  28. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  29. "The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1."Science 320:1496-1501(2008) [PubMed] [Europe PMC] [Abstract]
  30. "DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival."Cell 137:873-886(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Site-specific mTOR phosphorylation promotes mTORC1-mediated signaling and cell growth."Mol. Cell. Biol. 29:4308-4324(2009) [PubMed] [Europe PMC] [Abstract]
  32. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  33. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  34. "mTOR phosphorylated at S2448 binds to raptor and rictor."Amino Acids 38:223-228(2010) [PubMed] [Europe PMC] [Abstract]
  35. "Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids."Cell 141:290-303(2010) [PubMed] [Europe PMC] [Abstract]
  36. "DAP1, a novel substrate of mTOR, negatively regulates autophagy."Curr. Biol. 20:1093-1098(2010) [PubMed] [Europe PMC] [Abstract]
  37. "A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability."Genes Dev. 24:1939-1950(2010) [PubMed] [Europe PMC] [Abstract]
  38. "Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes."Genes Dev. 24:2019-2030(2010) [PubMed] [Europe PMC] [Abstract]
  39. "Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly."J. Biol. Chem. 285:20109-20116(2010) [PubMed] [Europe PMC] [Abstract]
  40. "mTORC1 directly phosphorylates and regulates human MAF1."Mol. Cell. Biol. 30:3749-3757(2010) [PubMed] [Europe PMC] [Abstract]
  41. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  42. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  43. "mTOR kinase domain phosphorylation promotes mTORC1 signaling, cell growth, and cell cycle progression."Mol. Cell. Biol. 31:2787-2801(2011) [PubMed] [Europe PMC] [Abstract]
  44. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  45. "The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling."Science 332:1317-1322(2011) [PubMed] [Europe PMC] [Abstract]
  46. "5-HT(6) receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia."EMBO Mol. Med. 4:1043-1056(2012) [PubMed] [Europe PMC] [Abstract]
  47. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  48. "Phosphorylation of the TOR ATP binding domain by AGC kinase constitutes a novel mode of TOR inhibition."J. Cell Biol. 203:595-604(2013) [PubMed] [Europe PMC] [Abstract]
  49. "Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains."PLoS ONE 8:E65586-E65586(2013) [PubMed] [Europe PMC] [Abstract]
  50. "Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis."Science 339:1320-1323(2013) [PubMed] [Europe PMC] [Abstract]
  51. "Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1."Science 339:1323-1328(2013) [PubMed] [Europe PMC] [Abstract]
  52. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  53. "SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1."Nature 519:477-481(2015) [PubMed] [Europe PMC] [Abstract]
  54. "Metabolism. Lysosomal amino acid transporter SLC38A9 signals arginine sufficiency to mTORC1."Science 347:188-194(2015) [PubMed] [Europe PMC] [Abstract]
  55. "Structure of the FKBP12-rapamycin complex interacting with the binding domain of human FRAP."Science 273:239-242(1996) [PubMed] [Europe PMC] [Abstract]
  56. "Refined structure of the FKBP12-rapamycin-FRB ternary complex at 2.2 A resolution."Acta Crystallogr. D 55:736-744(1999) [PubMed] [Europe PMC] [Abstract]
  57. "Insights into the domain and repeat architecture of target of rapamycin."J. Struct. Biol. 170:354-363(2010) [PubMed] [Europe PMC] [Abstract]
  58. "Structure of the human mTOR complex I and its implications for rapamycin inhibition."Mol. Cell 38:768-774(2010) [PubMed] [Europe PMC] [Abstract]
  59. "mTOR kinase structure, mechanism and regulation."Nature 497:217-223(2013) [PubMed] [Europe PMC] [Abstract]
  60. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  61. "Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma." Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract]