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Deoxyribonuclease I (DNASE1)

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DNaseI; DNL1; DRNI; DNase-I; Dornase alfa

Deoxyribonuclease I (DNASE1)
CCC B127
HGNC 2956
MGI 103157
PubMed 11479590
RGD 2510
UniProt P24855
Wiki DNASE1

DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB127Hu01 Recombinant Deoxyribonuclease I (DNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
APB127Hu01 Active Deoxyribonuclease I (DNASE1) Cell culture; Activity Assays.
Antibodies PAB127Hu01 Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC
MAB127Hu22 Monoclonal Antibody to Deoxyribonuclease I (DNASE1) WB,IHC
LAB127Hu82 FITC-Linked Monoclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IF.
LAB127Hu72 Biotin-Linked Monoclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC.
LAB127Hu71 Biotin-Linked Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC.
LAB127Hu81 FITC-Linked Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IF.
MAB127Hu21 Monoclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
Assay Kits SEB127Hu ELISA Kit for Deoxyribonuclease I (DNASE1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB127Mu01 Recombinant Deoxyribonuclease I (DNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB127Mu02 Recombinant Deoxyribonuclease I (DNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB127Mu01 Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
PAB127Mu02 Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
Assay Kits SEB127Mu ELISA Kit for Deoxyribonuclease I (DNASE1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxyribonuclease I (DNASE1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxyribonuclease I (DNASE1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyribonuclease I (DNASE1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyribonuclease I (DNASE1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyribonuclease I (DNASE1) ELISA Kit Customized Service Offer

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB127Bo01 Recombinant Deoxyribonuclease I (DNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB127Bo01 Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
MAB127Bo21 Monoclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
Assay Kits SEB127Bo ELISA Kit for Deoxyribonuclease I (DNASE1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum."Proc. Natl. Acad. Sci. U.S.A. 87:9188-9192(1990) [PubMed] [Europe PMC] [Abstract]
  2. "Structure of the human deoxyribonuclease I (DNase I) gene: identification of the nucleotide substitution that generates its classical genetic polymorphism."Ann. Hum. Genet. 59:1-15(1995) [PubMed] [Europe PMC] [Abstract]
  3. "DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis."Eur. J. Immunol. 31:743-751(2001) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation."FEBS J. 273:3094-3105(2006) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I."J. Exp. Med. 168:1767-1779(1988) [PubMed] [Europe PMC] [Abstract]
  9. "Human genetically polymorphic deoxyribonuclease: purification, characterization, and multiplicity of urine deoxyribonuclease I."J. Biochem. 108:393-398(1990) [PubMed] [Europe PMC] [Abstract]
  10. "Mutation of DNASE1 in people with systemic lupus erythematosus."Nat. Genet. 28:313-314(2001) [PubMed] [Europe PMC] [Abstract]
  11. "Molecular analysis of the third allele of human deoxyribonuclease I polymorphism."Ann. Hum. Genet. 59:139-147(1995) [PubMed] [Europe PMC] [Abstract]
  12. "The molecular basis for genetic polymorphism of human deoxyribonuclease I: identification of the nucleotide substitution that generates the fourth allele."FEBS Lett. 359:211-214(1995) [PubMed] [Europe PMC] [Abstract]
  13. "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism."Electrophoresis 18:1936-1939(1997) [PubMed] [Europe PMC] [Abstract]
  14. "A new allele, DNASE1*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability."Biochem. Biophys. Res. Commun. 260:280-283(1999) [PubMed] [Europe PMC] [Abstract]