Wide-range ELISA Kit for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- Product No.WEB070Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Tryptase (TPS).
No significant cross-reactivity or interference between Wide-range Tryptase (TPS) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Tryptase (TPS) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Tryptase (TPS) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Tryptase (TPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Tryptase (TPS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Allergy and Clinical Immunology||Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps PubMed: 22534535|
|J Pediatr.||Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 Pubmed:24530123|
|Mediators of Inflammation||Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334|
|PLOS ONE||A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats Pubmed:25541997|
|Neurogastroenterology & Motility||Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271|
|Mediators Inflamm||Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report PubMed: 26089601|
|Int Immunopharmacol||Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis PubMed: 26049029|
|Mediators of Inflammation||Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins Journals: Mi|
|Mediators of Inflammation||Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins Pubmed:27057098|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB070Hu01||Recombinant Tryptase (TPS)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB070Hu01||Polyclonal Antibody to Tryptase (TPS)||WB; IHC; ICC; IP.|
|LAB070Hu71||Biotin-Linked Polyclonal Antibody to Tryptase (TPS)||WB; IHC; ICC.|
|MAB070Hu24||Monoclonal Antibody to Tryptase (TPS)||WB; IHC; ICC; IP.|
|MAB070Hu26||Monoclonal Antibody to Tryptase (TPS)||WB; IHC; ICC; IP.|
|MAB070Hu22||Monoclonal Antibody to Tryptase (TPS)||WB; IHC; ICC; IP.|
|LAB070Hu76||Biotin-Linked Monoclonal Antibody to Tryptase (TPS)||WB; IHC; ICC.|
|WEB070Hu||Wide-range ELISA Kit for Tryptase (TPS)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEB070Hu||ELISA Kit for Tryptase (TPS)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCB070Hu||CLIA Kit for Tryptase (TPS)||Chemiluminescent immunoassay for Antigen Detection.|
|PSB070Hu01||Antibody Pair for Tryptase (TPS)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSB070Hu01||ELISA Kit DIY Materials for Tryptase (TPS)||Main materials for "Do It (ELISA Kit) Yourself".|