Wide-range ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)
NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
- Product No.WEA837Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.065ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Nitric Oxide Synthase 2, Inducible (NOS2).
No significant cross-reactivity or interference between Wide-range Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Nitric Oxide Synthase 2, Inducible (NOS2) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Nitric Oxide Synthase 2, Inducible (NOS2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Nitric Oxide Synthase 2, Inducible (NOS2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease||High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice PubMed: 20713153|
|Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease||Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression ScienceDirect: S0925443911001323|
|FEBS Journal||Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta Wiley: source|
|Gene||Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors ScienceDirect: S0378111911007347|
|PLoS One||A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide PubMed: 23359814|
|PLoS ONE||Regulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways PlosOne: Source|
|Journal of Cellular Biochemistry||The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis. PubMed: 22234777|
|International Immunopharmacology||Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114|
|Integrative Cancer Therapies||Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits Pubmed: 24013642|
|Food and Chemical Toxicology||Inhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 mice Pubmed: 23567245|
|Experimental Gerontology||Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion ScienceDirect: S0531556513003331|
|International Immunopharmacology||Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits ScienceDirect: S1567576913004153|
|INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH||L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source|
|Molecular Vision||Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats Pubmed:24940038|
|Journal of Environmental Pathology, Toxicology and Oncology||Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways Pubmed:24941292|
|Eur J Cancer Prev.||Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies Pubmed:25222193|
|Cell and Tissue Research||Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes Pubmed:25673209|
|Ann N Y Acad Sci.?||Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis Pubmed:5243883|
|Molecular Medicine Reports||Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury PubMed: 26458999|
|Chem Biol Interact||Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4 PubMed: 26522475|
|Journal of Environmental Pathology, Toxicology and Oncology||Protective Effect of PubMed: 26349607|
|Journal of Immunotoxicology||Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2 PubMed: 25738525|
|Cancer Medicine||Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254|
|Journal of Natural Medicines||Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice Pubmed:26972100|
|Cell Mol Immunol||Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496|
|Медицина и здравоохранение||Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу article:n|
|Graefe's Archive for Clinical and Experimental Ophthalmology||Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909|
|Taylor & Francis||Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494|
|Molecular Vision||Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats. pubmed:27440992|
|Journal of Natural Medicines||Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice. pubmed:26972100|
|Applied Biochemistry and Biotechnology||Protective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines. pubmed:27435276|