Wide-range ELISA Kit for Thrombospondin 1 (THBS1)
- Product No.WEA611Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeplasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.244ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Thrombospondin 1 (THBS1).
No significant cross-reactivity or interference between Wide-range Thrombospondin 1 (THBS1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Thrombospondin 1 (THBS1) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Thrombospondin 1 (THBS1) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||87-95||91|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Thrombospondin 1 (THBS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Thrombospondin 1 (THBS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Thrombospondin 1 (THBS1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||83-97%||96-103%||86-96%||93-101%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Physiology and Biochemistry||Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats SpringerLink: 1v552w21p7423764|
|Basic Research in Cardiology||Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170|
|PloS one||Anthocyanin-Rich Purple Corn Extract Inhibit Diabetes-Associated Glomerular Angiogenesis Pubmed: 24278186|
|BMC Complementary and Alternative Medicine||Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121|
|Oncotarget||Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay pubmed:26993605|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA611Mu02||Recombinant Thrombospondin 1 (THBS1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA611Mu01||Recombinant Thrombospondin 1 (THBS1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA611Mu03||Recombinant Thrombospondin 1 (THBS1)||Positive Control; Immunogen; SDS-PAGE; WB.|
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|PAA611Mu03||Polyclonal Antibody to Thrombospondin 1 (THBS1)||WB; IHC; ICC; IP.|
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|WEA611Mu||Wide-range ELISA Kit for Thrombospondin 1 (THBS1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
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