Wide-range ELISA Kit for Immunoglobulin A (IgA)
- Product No.WEA546Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample Typeserum, plasma, and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.4ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 722
For more details, please contact local distributors! US$ 3249
For more details, please contact local distributors! US$ 6137
For more details, please contact local distributors! US$ 50540
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Wide-range Immunoglobulin A (IgA).
No significant cross-reactivity or interference between Wide-range Immunoglobulin A (IgA) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Immunoglobulin A (IgA) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Immunoglobulin A (IgA) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Immunoglobulin A (IgA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Immunoglobulin A (IgA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Immunoglobulin A (IgA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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|international Journal of food and nutritional sciences||EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source|
|Poultry Science||Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 Pubmed: 24570422|
|Clinical and Vaccine Immunology||Specific humoral immune response induced by Propionibacterium acnes can prevent Actinobacillus pleuropneumoniae infection in mice Asm: Source|
|Clin Vaccine Immunol.||Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice Pubmed:24429068|
|Journal of Proteomics||Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection Pubmed:24576640|
|Food & Function||Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407|
|Pediatrics||Oropharyngeal colostrum administration in extremely premature infants: an RCT. Pubmed:25624376|
|Front Microbiol||Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis PubMed: 26217323|
|Food and Agricultural Immunology||Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers 10.108:09540105.2017.1311308|
|SYSTEMISCHE ENTZÜNDUNG BEI CHRONISCHER NIERENINSUFFIZIENZ – DIE ROLLE DER DARMFLORA patent:US9763998B2|
|Water Research||Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095|
|Vaccine||Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens Pubmed:29289381|
|Food and Agricultural Immunology||Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers 10.1080:09540105.2018.1457013|
|Probiotics Antimicrob Proteins||Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … Pubmed:29305686|
|SAGE Open Medicine||Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes Pubmed:29623201|
|Nephrology Dialysis Transplantation||The gut flora modulates intestinal barrier integrity but not progression of chronic kidney disease in hyperoxaluria-related nephrocalcinosis Pubmed: 31081025|
|Current Developments in Nutrition||Yacon (Smallanthus sonchifolius): Effect on Integrity of Intestinal Barrier, Inflammatory Response and Oxidative Stress in Animal Model of Colon Cancer (P06-052-19) Pubmed: 31225040|
|Nutrients.||Effects of Paramylon Extracted from Euglena gracilis EOD-1 on Parameters Related to Metabolic Syndrome in Diet-Induced Obese Mice Pubmed: 31330894|
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