ELISA Kit for Eosinophil Peroxidase (EPX)

EPO; EPP; EPX-PEN; EPER

Specificity

This assay has high sensitivity and excellent specificity for detection of Eosinophil Peroxidase (EPX).
No significant cross-reactivity or interference between Eosinophil Peroxidase (EPX) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Eosinophil Peroxidase (EPX) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Peroxidase (EPX) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 89-101 93
heparin plasma(n=5) 90-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Peroxidase (EPX) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Peroxidase (EPX) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Peroxidase (EPX) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 92-101% 81-93% 80-96%
EDTA plasma(n=5) 83-104% 83-101% 79-103% 84-95%
heparin plasma(n=5) 80-89% 78-93% 88-95% 80-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
International archives of allergy and immunology Allergen-Induced Increases in Interleukin-25 and Interleukin-25 Receptor Expression in Mature Eosinophils from Atopic Asthmatics. pubmed:27685606
Experimental and Therapeutic Medicine Eosinophilic biomarkers for detection of acute exacerbation of chronic obstructive pulmonary disease with or without pulmonary embolism 10.3892/etm.2017.4876
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPJ138Mu01 Recombinant Eosinophil Peroxidase (EPX) Positive Control; Immunogen; SDS-PAGE; WB.
EPJ138Mu61 Eukaryotic Eosinophil Peroxidase (EPX) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ138Mu02 Recombinant Eosinophil Peroxidase (EPX) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ138Mu01 Polyclonal Antibody to Eosinophil Peroxidase (EPX) WB; IHC; ICC; IP.
PAJ138Mu02 Polyclonal Antibody to Eosinophil Peroxidase (EPX) WB; IHC; ICC; IP.
SEJ138Mu ELISA Kit for Eosinophil Peroxidase (EPX) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ138Mu Multiplex Assay Kit for Eosinophil Peroxidase (EPX) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.