ELISA Kit for Carbonic Anhydrase VI (CA6)

CA-VI; CAVI; GUSTIN; Carbonate dehydratase VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase

Specificity

This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase VI (CA6).
No significant cross-reactivity or interference between Carbonic Anhydrase VI (CA6) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Carbonic Anhydrase VI (CA6) and the recovery rates were calculated by comparing the measured value to the expected amount of Carbonic Anhydrase VI (CA6) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carbonic Anhydrase VI (CA6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 79-91% 80-101% 87-99%
EDTA plasma(n=5) 80-97% 92-101% 90-98% 87-99%
heparin plasma(n=5) 88-101% 83-97% 89-103% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Metabolomics Salivary markers of taste sensitivity to oleic acid: a combined proteomics and metabolomics approach Springer: Source
American Journal of Immunology SALIVARY CARBONIC ANHYDRASE VI, ZINC SULFATE TASTE ACUITY AND FREQUENCY OF ILLNESS: A PILOT STUDY Com:Source
Physiol Behav.  Associations between food consumption patterns and saliva composition: Specificities of eating difficulties children. pubmed:28185876
Chemical Senses Differences in the density of fungiform papillae and composition of saliva in patients with taste disorders compared to healthy controls pubmed:28981826
Caries research Children with a Higher Activity of Carbonic Anhydrase VI in Saliva Are More Likely to Develop Dental Caries. pubmed:28633135
Scientific Reports Obese Subjects With Specific Gustatory Papillae Microbiota and Salivary Cues Display an Impairment to Sense Lipids Pubmed:29713004
Appetite Acceptance of added fat to first complementary feeding purees: An exploration of fat type, feeding history and saliva composition Pubmed: 30172680
International journal of molecular sciences Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries Pubmed: 31151296
Acta Odontol Scand Polymorphism in the CAVI gene, salivary properties and dental caries Pubmed: 32013665
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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