ELISA Kit for Vanin 1 (VNN1)

Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Vanin 1 (VNN1).
No significant cross-reactivity or interference between Vanin 1 (VNN1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Vanin 1 (VNN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vanin 1 (VNN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-101 96
EDTA plasma(n=5) 93-105 98
heparin plasma(n=5) 84-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vanin 1 (VNN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vanin 1 (VNN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vanin 1 (VNN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 98-105% 85-99% 94-101%
EDTA plasma(n=5) 97-105% 87-95% 81-89% 83-99%
heparin plasma(n=5) 90-102% 79-95% 85-101% 79-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Toxicology Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury ScienceDirect: S0300483X11003234
Journal of Pharmacology and Experimental Therapeutics Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury Aspet: 192807
J Pharmacol Exp Ther Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury. PubMed: 22399813
Journal of Applied Toxicology Early detection of renal injury using urinary vanin-1 in rats with experimental colitis PubMed: 23307618
Iranian Journal of Health and Physical Activity Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats Jm.um: Source
Platelets Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia Pubmed: 23534352
Journal of Applied Toxicology Early detection of renal injury using urinary vanin-1 in rats with experimental colitis. Pubmed: 23307618
Diabetes &amp; Vascular Disease Research Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats Sagepub:Source
Food Chem Toxicol. The effect of activated charcoal on adenine-induced chronic renal failure in rats Pubmed:24412558
Hypertension Research Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake PubMed: 26376947
Inflammation Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance PubMed: 26253296
Toxicology Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma Pubmed:27317936
J Biomed Mater Res A Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice Pubmed:26850371
APMIS Citrate attenuates vascular calcification in chronic renal failure rats. pubmed:28332248
Clin Exp Pharmacol Physiol. Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. pubmed:29027259
Cellular Physiology and Biochemistry Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … Pubmed:29550811
International Journal of Molecular Sciences Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis Pubmed: 30332759
International Journal of Molecular Sciences Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats Pubmed: 31514290
The Journal of Clinical Hypertension homepage Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study Pubmed: 32869948
J Clin Hypertens Association of urinary vanin©\1 with kidney function decline in hypertensive patients 34029440
Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study 33624223
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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